Objective: To determine the content of hypoxanthine in Pheretima aspergillum from different habitats.
Method: A RP-HPLC method was established. The chromatographic column was Inertsil ODS-EP. The mobile phase was H2O-CH3OH-C4H8O(93:: 7: 0.05). The flow rate was 1.0 ml/min, and the detection wavelength was 254 nm.
Results: The average recoveries for hypoxanthine was 98.6% , precision of the method was 0. 50% (RSD, n = 6).
Conclusion: The method can be used to determine the content of hypoxanthine in Pheretima aspergillum from diffrent habitats.
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