Purpose: To better understand the molecular mechanisms underlying the radio-protective effect of soybean isoflavone that we observed in our recent animal experiments.
Materials And Methods: We utilized a cDNA microarray to investigate the expression profiles of 4,096 known genes in the livers of irradiated-mice with or without soybean isoflavone treatment. Dye swap approach was employed to control for gene-specific dye bias and quantitative real-time RT-PCR was performed on several genes to validate the cDNA microarray data.
Results: Compared with the control group, 68 genes were up-regulated and 28 genes were down-regulated in mice treated with irradiation alone, whereas only 6 genes were down-regulated and 35 genes were up-regulated in mice treated with soybean isoflavone. Interestingly, some of the down-regulated genes in the irradiated group, such as DNA repair and stress response genes and cytoskeleton-associated genes, which are markers of cellular damage after irradiation, were maintained at close to normal expression levels after soybean isoflavone treatment.
Conclusions: Comparison of gene expression profiles in the livers of irradiated-mice treated with or without soybean isoflavone suggested that soybean isoflavone may be an efficient tool to reverse irradiation damage of the liver through multiple-pathways and also provides important clues to further pursue the molecular mechanisms underlying the radio-protective activity of soybean isoflavone.
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