We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 A) than any previously obtained with biarsenical dyes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/ja063212q | DOI Listing |
Publication Analysis
Top Keywords
biarsenical dyes
8
improved photostable
4
photostable fret-competent
4
fret-competent biarsenical-tetracysteine
4
biarsenical-tetracysteine probes
4
probes based
4
based fluorinated
4
fluorinated fluoresceins
4
fluoresceins developed
4
developed fluoro-substituted
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!