AI Article Synopsis

  • The study examines how polyphenol oxidase (PPO) activity and substrates affect the quality of durum wheat.
  • A molecular-marker linkage map was created using recombinant inbred lines from two durum wheat cultivars, leading to the identification of quantitative trait loci (QTL) related to PPO activity.
  • The findings suggest that lower PPO activity, linked to specific markers on chromosome 2A, can be targeted for improvement through marker-assisted selection in durum wheat breeding.

Article Abstract

The quality of durum wheat (Triticum turgidum ssp. durum) is influenced by polyphenol oxidase(PPO) activity and its corresponding substrates. A saturated molecular-marker linkage map was constructed previously by using a set of recombinant inbred (RI) lines, derived from a cross between durum wheat cultivars Jennah Khetifa and Cham 1. Quantitative trait loci (QTL) for PPO activity in seeds were mapped in this population. PPO activity in seeds of the parents and 110 RI lines was measured spectrophotometrically. The PPO activity of Cham 1 was significantly lower than that of Jennah Khetifa. QTL analysis of these data indicated that most of PPO activity was associated with major loci on the long arm of chromosome 2A. The trait was found to be strongly associated with the SSR marker Xgwm312@2A. With this knowledge, marker-assisted selection can be used to select genotypes with lower PPO activity in durum wheat populations.

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Source
http://dx.doi.org/10.1007/BF03194624DOI Listing

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