Immunocytochemistry has emerged as a powerful research tool in neurobiology. One of the widely used methods is an indirect fluorescence technique that uses FITC- conjugated IgG to visualise protein expression within tissues, but a major drawback of this technique is the high background fluorescence due to non-specific antibody binding. Gut innervation is complex and best visualized in three-dimensions in whole mount preparations. We describe a simple and easy to use counterstaining procedure in conjunction with an indirect immunofluorescence technique in gut whole mount preparations that largely eliminates background fluorescence and creates a contrasting background against the bright antigen-antibody complexes. Furthermore, this technique allows the detailed qualitative and quantitative study of myenteric plexuses in whole-mount preparations.
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Article Synopsis
- The mammary gland experiences significant structural and compositional changes throughout a woman's life, mainly influenced by hormones and environmental factors, including phases like pregnancy and menopause.
- Research using rodent models has been helpful in understanding these processes but doesn't completely represent human development, leading to interest in using rabbits as alternative models due to their similarities to human mammary biology.
- A new methodology has been developed for preparing and analyzing rabbit mammary gland whole mounts, addressing the technical challenges of thicker tissue and paving the way for better research on mammary gland growth and the impacts of hormonal or toxicant disruptions.
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