AI Article Synopsis
- The study aimed to enhance the sensitivity of the rabies genome hybridization test by using PCR amplification after reverse transcription of rabies RNA extracted from infected brain tissue.
- The presence of the amplified DNA was confirmed using cDNA synthesized from either an antigenomic primer or an antimessenger primer.
- This method could lead to more accurate detection of rabies in infected samples.
Article Abstract
In an attempt to improve the sensitivity of the rabies genome hybridization test, PCR amplification was used following reverse transcription of rabies RNA extracted from infected brain. Presence of amplified DNA is demonstrated with either cDNA synthesized from the antigenomic primer or from antimessenger primer.
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| http://dx.doi.org/10.1016/0890-8508(90)90052-2 | DOI Listing |
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