AI Article Synopsis
- Leukocyte adhesion to blood vessel lining is crucial in many inflammatory diseases.
- Recent advancements have used atomic force microscopy (AFM) to study how leukocytes interact with endothelial cells, specifically focusing on cell adhesion force unbinding curves.
- A custom-built laser tweezers system measured the force interactions between living THP-1 cells and HUVEC monolayers, uncovering details about force relationships and cell detachment, while suggesting further studies on the time-related aspects of cell behavior.
Article Abstract
Leukocyte adhesion to vascular endothelium is an initial step of many inflammatory diseases. Although the atomic force microscopy (AFM) measurements of leukocyte-endothelial interaction have been recently introduced. with cell adhesion force unbinding curves (CAFUC). We obtained pico-Newton force in the initial interaction between a single living THP-1 cell and HUVEC monolayer using a custom-built laser tweezers (LT) system. The measured quantities included the non-linear force-distance relationship, and the effect of yielding in cell detachment. It is possible to introduce a time scale into the LT cell-detachment experiments for further exploration and more detailed information on the viscoelastic properties of living cells.
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| http://dx.doi.org/10.1007/s10558-006-9012-6 | DOI Listing |
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