Quantification of cyanamide contents in herbaceous plants.

Biosci Biotechnol Biochem

Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Kami-ina, Nagano, Japan.

Published: September 2006

AI Article Synopsis

  • Cyanamide (NH2CN) is a naturally occurring compound primarily recognized for its industrial use, and its presence in the plant kingdom was studied using a precise method called stable isotope dilution gas chromatography-mass spectrometry (SID-GC-MS).
  • The SID-GC-MS method successfully quantified cyanamide levels in 101 species of herbaceous plants, with an average recovery rate of about 55.6%.
  • Among the plants tested, only Vicia villosa and V. cracca showed significant cyanamide concentrations, while the other 99 species had undetectable levels, indicating that cyanamide distribution in plants is both limited and uneven.

Article Abstract

Cyanamide (NH2CN) is found in nature, although it has long been recognized as an industrial product. Distribution of cyanamide in the plant kingdom was investigated using a direct quantitative determination method to detect and measure cyanamide by stable isotope dilution gas chromatography-mass spectrometry (the SID-GC-MS method). The SID-GC-MS method proved to be a robust way to quantify cyanamide contents in the extracts of 101 species of herbaceous plants. The average recovery of cyanamide from all plants tested was 55.6+/-20.3%. Vicia villosa and V. cracca contained cyanamide at 369-498 microg/gFW and 3,460-3,579 microg/gFW respectively, while the other 99 species contained no detectable cyanamide (<1 microg/gFW). This result suggests that distribution of cyanamide in the plant kingdom is limited and uneven.

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Source
http://dx.doi.org/10.1271/bbb.60171DOI Listing

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