The article compares the ability of detection and detection times (TTD) of aerobic bacteria in the BacT/Alert FA, BacT/Alert SA, Bactec Plus Aerobic/F and Bactec Standard Aerobic/F bottles. Compared bottles were inoculated at the same time with the identical suspension of bacterial strain. All bottles detected bacterial pathogens even in the case of very low number of inoculated bacteria. The differences of TTD were detected between the bottles of compared hemocultivation systems. Bactec Plus Aerobic/F system is faster in the detection of the members of family Enterobacteriaceae at 1-3 hours, BacT/Aert FA system is faster in the detection of coagulase-negative staphylococci. Most difference was detected at Pseudomanas stutzeri, where the detection in BacT/Alert FA bottles was at 15 hours faster in opposite of Bactec Plus Aerobic/F bottles. In the bottles without sorbent Staphylococcus aureus and Streptococcus sanqui were detected faster in the system Bactec Standard Aerobic/F. The bottles BacT/Alert SA detected faster skin corynebacteria. Detected differences have not a practical importance for the blood stream infection diagnostics.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Experimental study of specific and nonspecific blood culture bottles for the diagnosis of candidemia.
Rev Iberoam Micol
October 2024
Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul (RS), Brazil; Mycology Laboratory of FAMED-FURG, Rio Grande, Rio Grande do Sul (RS), Brazil. Electronic address:
Background: Early diagnosis of candidemia is critical for the correct management and treatment of patients.
Aims: To test the efficacy of different blood culture bottles in the growth of Candida strains.
Methods: We compared the performance of BD BACTEC™ Plus Aerobic/F (Aero) culture bottles with the specific BD BACTEC™ Mycosis IC/F Lytic (Myco) culture bottles using the BD BACTEC™ FX 40 automated blood culture system to determine the mean time-to-detection (TTD) in Candida species.
The effect of taurolidine on the time-to-positivity of blood cultures.
Infect Prev Pract
June 2024
Department of Medical Microbiology, University Medical Centre Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.
Article Synopsis
- Taurolidine lock solutions (TL) may prevent bloodstream infections but can interfere with blood culture results if not fully aspirated before testing.
- Various lock solutions were tested for their impact on microbial detection in blood culture vials, with taurolidine showing a significant delay in detecting microbial growth.
- The findings emphasize the necessity of removing TLs from central venous catheters prior to collecting blood cultures to avoid misleading results.
Evaluation of Different Blood Culture Bottles for the Diagnosis of Bloodstream Infections in Patients with HIV.
Infect Dis Ther
November 2023
Department of Infectious Diseases, Wenzhou Central Hospital, The Dingli Clinical College of Wenzhou Medical University, The Second Affiliated Hospital of Shanghai University, Zhejiang, 325000, China.
Introduction: Bloodstream infection (BSI) is a significant factor contributing to hospitalization and high mortality rates among human immunodeficiency virus(HIV)-positive patients. Therefore, the timely detection of this condition is of utmost importance. Blood culture is considered the gold standard for diagnosing BSIs.
View Article and Find Full Text PDFDifferent BD BACTEC Blood Culture Bottle Types for the Detection of Fungi in Simulated Sterile Body Fluid Samples.
Diagnostics (Basel)
May 2023
Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška Cesta 4, SI-1000 Ljubljana, Slovenia.
Blood culture systems are a potential alternative to classical cultivation of fungi on mycological media, but there are limited data on the suitability of these systems for culturing other sample types (e.g., sterile body fluids).
View Article and Find Full Text PDFSubculturing and Gram staining of blood cultures flagged negative by the BACTEC™ FX system: Optimizing the workflow for detection of in clinical specimens.
Front Microbiol
March 2023
Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Objective: To investigate whether an incubation time of 5 days (Aerobic/F, Anaerobic/F) and 14 days (Myco/F) blood culture bottles is sufficient to prevent false-negative results.
Methods: We evaluated 1,244 blood bottles (344 patients) defined as negative by the BACTEC™ FX system. We also reviewed published cases and our own cases of bloodstream infection caused by and simulated different scenarios, including different inoculation concentrations, bottle types, and clinical isolates.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!