Production kinetics and tensioactive characteristics of biosurfactant from a Pseudomonas aeruginosa mutant grown on waste frying oils.

Biotechnol Lett

Polymer Chemistry Laboratory, National Centre of Excellence in Physical Chemistry, University of Peshawar, Peshawar, 25120, Islamic Republic of Pakistan.

Published: October 2006

Various waste frying oils (WFOs) were evaluated as substrates for rhamnolipid production by Pseudomonas aeruginosa mutant EBN-8 in the presence or absence of rhamnolipid precursor, under single-/batch-fed conditions. Soybean WFO was the best substrate, producing 9.3 g rhamnolipid l(-1) with the specific product yield of 2.7 g g(-1) h, under batch-fed cultivation with the addition of rhamnolipid precursor. The surface tension of the cell-free culture broth (CFCB) was 29.1 mN m(-1 )and the interfacial tension against n-hexadecane was <1 mN m(-1). The hydrocarbon/ CFCB systems showed the relative emulsion stability to be in the range of 89.7-92.3.

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http://dx.doi.org/10.1007/s10529-006-9134-3DOI Listing

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