The objectives of this study were (a) to construct an in vitro model of rabbit dural healing, (b) to test the influence of collagen, laminin, and poly-L-lysine on the migration and proliferation of dural cells, and (c) to study the healing mechanism of duraplasty. Rabbit dural pieces (1.5 cm x 1.5 cm) were perforated in their central part with a 2 mm punch to mimic a dural defect. The dural pieces were cultured in 24-well plates that had been coated with collagen, laminin, or poly-L-lysine, and the influence of different extracellular matrices on migration and proliferation of dural cells was observed. Cells were subcultured on slides for immunocytochemistry to study their characteristics; dural healing was observed by scanning electron microscopy. The results demonstrated that only the dural pieces that were cultured on collagen-coated wells showed migration of cells into the central defect after a period of 8 to 10 days and that healing of the dural defect occurred by 13 to 15 days. The cultured dural cells stained strongly positive with an antibody to vimentin, but negative with an antibody to factor VIII. New collagen fibers were observed in the dural defects. This report demonstrates that an in vitro model for dural healing was successfully constructed in collagen-coated wells; the results implicate cellular migration of fibroblasts from the dural defect margin as an important mechanism of wound healing following duraplasty.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Extrasinusoidal macrophages are a distinct subset of immunologically active dural macrophages.
Sci Immunol
December 2024
Institute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Although macrophages in the meningeal compartments of the central nervous system (CNS) have been comprehensively characterized under steady state, studying their contribution to physiological and pathological processes has been hindered by the lack of specific targeting tools in vivo. Recent findings have shown that the dural sinus and its adjacent lymphatic vessels act as a neuroimmune interface. However, the cellular and functional heterogeneity of extrasinusoidal dural macrophages outside this immune hub is not fully understood.
View Article and Find Full Text PDFAdvances in Research on Meningeal Lymphatic Vessels in Central Nervous System Diseases.
J Craniofac Surg
December 2024
Department of Neurosurgery, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang.
Article Synopsis
- - Meningeal lymphatic vessels (mLVs) are vital for draining fluids and waste from the brain and moving immune cells to lymph nodes, helping coordinate the brain's immune response with the rest of the body
- - Dysfunction of mLVs, especially with aging and various CNS diseases, can lead to toxic buildup in the brain, worsening conditions like neurodegenerative diseases and tumors
- - The review discusses the anatomy and roles of mLVs in CNS health and disease, suggesting that targeting these vessels with drugs or therapies could improve treatment outcomes for CNS disorders
Dural Tregs driven by astrocytic IL-33 mitigate depression through the EGFR signals in mPFC neurons.
Cell Death Differ
November 2024
Department of Pharmacology, Jiangsu Key Laboratory of Neurodegeneration, Nanjing Medical University, Nanjing, China.
The dura sinus-resident immune cells can influence the process of central neural system (CNS) diseases by communicating with central nerve cells. In clinical, Tregs are also frequently impaired in depression. However, the significance of this relationship remains unknown.
View Article and Find Full Text PDFCervical myelopathy caused by IgG4-related hypertrophic spinal pachymeningitis: Case report and a descriptive review of the literature.
Brain Spine
September 2024
Department of Neurosurgery, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany.
Article Synopsis
- IgG4-related disease is an immune disorder that can cause hypertrophic spinal pachymeningitis (HSP), leading to spinal cord and nerve root compression due to infiltration of IgG4-positive plasma cells in the meninges.* -
- A case study of a 45-year-old man with cervical myelopathy showed that surgical decompression and subsequent treatment with methylprednisolone and rituximab led to a full neurological recovery, highlighting effective management of this condition.* -
- An updated literature review covering 52 cases revealed that neurological impairment worsened in 58% of patients, with most lesions affecting the thoracic and cervical spine, indicating the complexities and challenges in diagnosing and treating IgG4-related H
Reimagining the meninges from a neuroimmune perspective: a boundary, but not peripheral.
J Neuroinflammation
November 2024
Department of Neurosurgery, Tianjin Medical University General Hospital, No.154, Anshan Road, Tianjin, 300052, P.R. China.
Recent advances in neuroscience have transformed our understanding of the meninges, the layers surrounding the central nervous system (CNS). Two key findings have advanced our understanding: researchers identified cranial bone marrow as a reservoir for meningeal immune cells, and rediscovered a brain lymphatic system. Once viewed merely as a protective barrier, the meninges are now recognized as a dynamic interface crucial for neuroimmune interactions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!