Seventeen agar samples were extracted from Gelidiella acerosa (Forsskal) Feldmann and Hamel (Rhodophyta, Gelidiales) specimens collected from nine different sites on the Indian coast-five from southeast coast and four from the west coast. The agar samples were analysed. The stability characteristics of the gels of selected agar samples were studied by rheometry under applied stress conditions, i.e. variation of the storage (G') and loss moduli (G'') were studied under varying frequency and duration (time) of the stress applied. Yield, apparent and dynamic viscosities, gelling and melting temperatures, 3,6-anhydrogalactose (3,6-AG), sulphate contents and TGA (Thermogravimetric Analysis) measurements of the products were done. It was observed that the best quality agar was produced by G. acerosa occurring in the Gulf of Mannar region in the southeast coast. The gel strengths and the viscosities of agars extracted from Gelidiella acerosa occurring in the Gulf of Mannar ranged from 500 to 700gcm(-2) and 33 to 45cP for 2001 collections and for 2002 collections the corresponding values were 450 to 845gcm(-2) and 55 to 67cP respectively. On the other hand, for the agar samples extracted from the west coast of India, the gel strength and viscosities values ranged from 225 to 400gcm(-2) and from 15 to 30cP, respectively. The agars obtained from G. acerosa collected from southeast coast have been found to be suitable for bacterial culture and molecular biology. This is the first report of superior quality of agar from the Indian agarophytes.
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http://dx.doi.org/10.1016/j.biortech.2006.07.028 | DOI Listing |
J Microbiol Methods
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National Food Institute, Technical University of Denmark, Henrik Dams Allé, 2800 Kgs. Lyngby, Denmark. Electronic address:
In the food industry, time-to-result is crucial for faster release of products, minimising recalls, mitigation of microbial contamination problems and, ultimately, food safety. Carrageenan is isolated from red seaweed (Rhodophyta) and applied in various foods and beverages as a gelling, thickening, texturing, or stabilizing agent due to its hygroscopic properties. Currently, the standard industry plate count method entails a one-hundred-fold dilution of the sample before mixing with molten agar for assessment of the level of microbial contamination in carrageenan samples prior to business-to-business shipment.
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Singapore Centre for Environmental Life Sciences Engineering (SCELSE), National University of Singapore, , Singapore.
Coastal water, sediment, and algae samples were collected from St. John's Island, Singapore, and enriched in either broth or agar. Metagenomic sequencing was carried out on DNA from these enrichments and analyzed.
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Department of Restorative and Prosthetic Dental Sciences, College of Dentistry, Dar Al Uloom University, Riyadh, Saudi Arabia.
Effect of different root canal irrigation regimes microbubble emulsion (MBE) via riboflavin photosensitizer (RFP), cerium oxide (CeO) nanoparticles (NPs), and Nd: YAP laser on antibacterial efficiency, microhardness (MH), smear layer (SL) removal efficacy, and push-out bond strength (PBS) of AH plus sealer to canal dentin. Sixty single-rooted teeth were selected, disinfected, and categorized into four groups based on the type of disinfection. Following disinfection, a pair of samples were randomly selected and visualized under scanning electron microscope (SEM) for SL evaluation.
View Article and Find Full Text PDFUnder stress, bacteria display selective growth propensities on solid and liquid media. For Mycobacterium tuberculosis (Mtb), differentially culturable bacteria have been found in tuberculosis (TB) patient sputa. We hypothesized that antibiotic treatment can induce selective culturability in Mtb.
View Article and Find Full Text PDFPlant Dis
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Universidad de Chile, Departamento de Sanidad Vegetal, Facultad de Ciencias Agronomicas, Casilla 1004, Santiago, Chile, 8820000;
Walnut (Juglans regia L.) is the primary nut tree cultivated in Chile, covering 44.626 ha.
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