The evaluation of simultaneous removal of carbon and nitrogen in an anaerobic inverse fluidized bed reactor is described. Continuous and batch experiments were used, with synthetic wastewater and glucose as the carbon source with two different nitrate concentrations of 100 and 250 mg N-NO3/L. The evolution of substrates and the concentrations of intermediary products in the gas phase were followed. Results indicate that the use of the biofilm in the inverse fluidized bed reactor allows the expression of denitrification and methanization activities simultaneously without physical or time separation. The removal of nitrogen with both the feeding of 100 and 250 mgN-NO3/L was higher than 90%, while the removal of carbon was 65% on average for the feeding with 100 mgN-NO3/L and 70% on average for the feeding with 250 mg N-NO3/L. This carbon degradation is equivalent to that obtained during the operation of the reactor in the period previous to the nitrate feeding. It was found that by using high values of the COD/N ratio, the dissimilative reduction of nitrates is favoured. Denitrification and anaerobic digestion occurs simultaneously under low values of COD/N.
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http://dx.doi.org/10.2166/wst.2006.493 | DOI Listing |
Adv Biotechnol (Singap)
February 2024
CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, 200030, China.
Pichia pastoris is a popular yeast host for high-level heterologous expression of proteins on an industrial scale owing to its reliable expression, robust growth, high fermentation density, and easy genetic manipulation and cultivation at a relatively low cost. Of particular interest is its high secretion efficiency for small proteins including insulin, human serum albumin, vaccines, enzymes, and llama-derived heavy-chain only antibodies (nanobodies) for pharmaceutical and research applications. However, a recurring challenge in using P.
View Article and Find Full Text PDFWater Sci Technol
January 2025
China Construction Fifth Engineering Division Co., Ltd, Changsha, Hunan 410004, China.
Road runoff underwent treatment using a filter filled with sludge from drinking water treatment plants to assess its capacity for removing dissolved organic matter (DOM). This evaluation utilized resin fractionation, gel permeation chromatography, three-dimensional excitation-emission matrix fluorescence spectroscopy, and UV-Visible spectroscopy. The filter demonstrated enhanced efficiency in removing dissolved organic carbon, achieving removal rates between 70 and 80%.
View Article and Find Full Text PDFBioengineered
December 2025
Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, India.
Polyhydroxyalkanoates (PHA) are bioplastics produced by few bacteria as intracellular lipid inclusions under excess carbon source and nutrient-deprived conditions. These polymers are biodegradable and resemble petroleum-based plastics. The rising environmental concerns have increased the demand for PHA, but the low yield in wild-type bacterial strains limits large-scale production.
View Article and Find Full Text PDFRSC Adv
January 2025
School of Materials Science and Engineering, Lanzhou University of Technology Lanzhou Gansu 730050 China.
Microbially induced calcium carbonate precipitation (MICP) has emerged as a promising technique for environmental remediation, particularly for heavy metal removal. This study explores the potential of MICP for Cr(iii) removal, analyzing the effects of temperature, pH, calcium source addition, and initial Cr(iii) concentration on removal efficiency. The results show that Cr(iii) can be efficiently removed with a removal rate approaching 100% under optimal conditions (25 °C, pH 7.
View Article and Find Full Text PDFRSC Adv
January 2025
Health Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences Tehran Iran
In addition to their advantages as promising methods for wastewater treatment, CWs exhibit poor performance in terms of N and P removal efficiency in the effluent of wastewater treatment plants. By focusing on this issue, we designed CWs integrated with a biochar-doped activated carbon cloth (ACC) electrode and alum sludge from water treatment plants as a substrate to achieve concomitant organic matter and nutrient removal efficiency. Compared with the use of one layer of alum sludge in CWs (CWs-C3) with ACC electrodes inserted in two layers, which uses one layer of alum sludge, a significant improvement in removal efficiency was achieved (96% for COD; 89% for TN; and 77% for TP).
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