The aim of the study was to identify at species level the Prevotella strains from 30 pus samples collected by needle aspiration in patients with abscesses of fascial spaces of the face and neck. Microscopy of Gram-stained direct smear and cultures on selective and nonselective media incubated aerobically and anaerobically were performed in each pus specimen. All strains of Gram-negative strictly anaerobic bacilli were identified to genus and species level using conventional methods of diagnosis and the Rapid ID 32 A system (BioMérieux, Marcy-l'Etoile, France). The Prevotella isolates were recovered (alone or in association with other bacteria) in 46.6% of the investigated specimens and were represented in reverse order of frequency by the following species: P. melaninogenica, P. denticola, P. oralis and P. loescheii. In conclusion, the rate of Prevotella isolation was relatively high and the most frequently isolated species was P. melaninogenica (followed by P. denticola), while no P. intermedia was found.
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