We report the activity, stability, and reusability of enzyme-carbon nanotube conjugates in aqueous solutions. A variety of enzymes were covalently attached to oxidized multi-walled carbon nanotubes (MWNTs). These conjugates were soluble in aqueous buffer, retained a high fraction of their native activity, and were stable at higher temperatures relative to their solution phase counterparts. Furthermore, the high surface area of MWNTs afforded high enzyme loadings, yet the intrinsic high length of the MWNT led to facile filtration. These water-soluble carbon nanotube-enzyme conjugates represent novel preparations that possess the virtues of both soluble and immobilized enzymes, thus providing a unique combination of useful attributes such as low mass transfer resistance, high activity and stability, and reusability.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/bit.21016 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The nucleolin antagonist N6L and paclitaxel combination treatment could be a new promising therapeutic strategy for pancreatic ductal adenocarcinoma therapy.
Eur J Pharmacol
January 2025
Université Paris-Est, Immunorégulation et Biothérapie, INSERM U955, Hôpital Henri Mondor, 94010 Créteil, France; AP-HP, Groupe hospitalo-universitaire Chenevier Mondor, Centre d'investigation clinique Biotherapie, F-94010 Creteil, France. Electronic address:
Pancreatic cancer (PCa) is one of the most devastating cancers with few clinical signs and no truly effective therapy. In recent years, our team has demonstrated that nucleolin antagonists such as N6L could be a therapeutic alternative for this disease. In order to study a possible clinic development of N6L (multivalent pseudopeptide), we undertook to study the effect of combination of N6L with chemotherapies classically used for PCa on the survival of pancreatic cancer cells.
View Article and Find Full Text PDFFullerene (C & C)-Meso-Tris-4-Carboxyphenyl Porphyrin Dyads Inhibit Entry of Wild-Type and Drug-Resistant HIV-1 Clades B and C.
J Med Virol
February 2025
Department of Chemistry, Assam University, Silchar, India.
The biological applications of noncationic porphyrin-fullerene (P-F) dyads as anti-HIV agents have been limited despite the established use of several cationic P-F dyads as anti-cancer photodynamic therapy (PDT) agents. This article explores the potential of amphiphilic non-cationic porphyrin-fullerene dyads as HIV-1 inhibitors under both PDT (light-treated) and non-PDT (dark) conditions. The amphiphilic P-F dyads, PBC and PBC, demonstrated enhanced efficacy in inhibiting the entry and production of HIV-1 (subtypes B and C).
View Article and Find Full Text PDFPreparation of curcumin submicron particles by supercritical antisolvent method with external adjustable annular gap nozzle.
Sci Rep
January 2025
Key Laboratory of High-efficiency and Clean Mechanical Manufacture (Ministry of Education), National Demonstration Center for Experimental Mechanical Engineering Education (Shandong University), School of Mechanical Engineering, Shandong University, Jinan, 250061, People's Republic of China.
The supercritical antisolvent (SAS) method can effectively improve the bioavailability of poorly water-soluble drugs. However, the current supercritical equipment and processes were not fully developed, making industrialization difficult to achieve. Therefore, an externally adjustable annular gap nozzle and its supporting equipment were designed.
View Article and Find Full Text PDFPhysicochemical properties and in vitro hypolipidemic activities of three different bonding state pectic polysaccharide fractions extracted sequentially from pear pulp.
Int J Biol Macromol
January 2025
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China. Electronic address:
In this study, water-soluble fraction (WSF), chelator-soluble fraction (CSF), and sodium carbonate-soluble fraction (NSF) were sequentially fractionated from pear pulp, of which physicochemical properties and hypolipidemic activities in vitro were evaluated. They showed distinct monosaccharide composition, surface morphology, nuclear magnetic resonance (NMR), and Fourier transform infrared (FT-IR) spectrums. WSF and NSF were identified as high methyl-esterified pectic polysaccharides with degrees of methyl esterification (DM) of 85.
View Article and Find Full Text PDFAerosol particles released from grit chambers of nine urban wastewater treatment plants in typical regions: Fugitive characteristics, quantitative drivers, and generation process.
Water Res
January 2025
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco- Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:
The flow through the grit chamber is non-biochemically treated wastewater, which contains microorganisms mainly from the source of wastewater generation. There are limited reports on aerosol particles generated by grit chambers compared with those produced by biochemical treatment tanks. This study analyzed the fugitive characteristics of aerosol particles produced in grit chambers at nine wastewater treatment plants in three regions of China.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!