The proliferative system of the small intestinal cryptic epithelium in intact mice in circahoralian rhythm of mitotic activity of its cells (with a 80-140-min period) is characterized by spatial and time organization. The characteristics of the studied proliferative system do not dub it in individual parts, but present new properties intrinsic of this system in general. The features are greatly similar to the features of spatial and time organization of this proliferative system in circadian rhythm of cell division.
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http://dx.doi.org/10.1007/s10517-006-0113-4 | DOI Listing |
Sci Rep
January 2025
Department of Robotics, Hanyang University, Ansan, 15588, Republic of Korea.
Diabetic retinopathy (DR) presents a significant concern among diabetic patients, often leading to vision impairment or blindness if left untreated. Traditional diagnosis methods are prone to human error, necessitating accurate alternatives. While various computer-aided systems have been developed to assist in DR detection, there remains a need for accurate and efficient methods to classify its stages.
View Article and Find Full Text PDFJ Immunoassay Immunochem
January 2025
JRF Department of Pathology, Jawaharlal Nehru Medical College, Datta Meghe Institute of Higher Education and Research, Wardha, India.
Background: The rising global burden of breast cancer demands early detection and effective treatment, with a focus on prognostic and predictive markers. The eighth edition of the American Joint Committee on Cancer staging manual introduced a new prognostic staging system to increase the predictive power of the existing anatomical staging system of breast cancer. The current study aimed to establish the correlation between Ki67 expression with molecular subtypes and with the pathological prognostic stage of invasive ductal carcinoma.
View Article and Find Full Text PDFBiochim Biophys Acta Mol Cell Res
January 2025
Designing Future Health Initiative, Center for Promotion of Innovation Strategy, Head Office of Enterprise Partnerships, Tohoku University, Miyagi 980-8579, Japan. Electronic address:
Proteasome-dependent protein degradation and the digestion of peptides by aminopeptidases are essential for myogenesis. Methionine aminopeptidases (MetAPs) are uniquely involved in, both, the proteasomal degradation of proteins and in the regulation of translation (via involvement in post-translational modification). Suppressing MetAP1 and MetAP2 expression inhibits the myogenic differentiation of C2C12 myoblasts.
View Article and Find Full Text PDFTissue Eng Part C Methods
January 2025
Trinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland.
Scaffold-free tissue engineering strategies using cellular aggregates, microtissues, or organoids as "biological building blocks" could potentially be used for the engineering of scaled-up articular cartilage or endochondral bone-forming grafts. Such approaches require large numbers of cells; however, little is known about how different chondrogenic growth factor stimulation regimes during cellular expansion and differentiation influence the capacity of cellular aggregates or microtissues to fuse and generate hyaline cartilage. In this study, human bone marrow mesenchymal stem/stromal cells (MSCs) were additionally stimulated with bone morphogenetic protein 2 (BMP-2) and/or transforming growth factor (TGF)-β1 during both monolayer expansion and subsequent chondrogenic differentiation in a microtissue format.
View Article and Find Full Text PDFNat Metab
January 2025
Department of Bioengineering, University of California, University of California, San Diego, La Jolla, CA, USA.
The Warburg effect, which describes the fermentation of glucose to lactate even in the presence of oxygen, is ubiquitous in proliferative mammalian cells, including cancer cells, but poses challenges for biopharmaceutical production as lactate accumulation inhibits cell growth and protein production. Previous efforts to eliminate lactate production in cells for bioprocessing have failed as lactate dehydrogenase is essential for cell growth. Here, we effectively eliminate lactate production in Chinese hamster ovary and in the human embryonic kidney cell line HEK293 by simultaneous knockout of lactate dehydrogenases and pyruvate dehydrogenase kinases, thereby removing a negative feedback loop that typically inhibits pyruvate conversion to acetyl-CoA.
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