J Exp Bot
Smurfit Institute of Genetics, Trinity College, Dublin 2, Ireland.
Published: December 2006
SU(VAR)3-9 proteins are key regulators of heterochromatin structure and function in plants, mammals, Drosophila, and yeast. In contrast to animals and fungi, plants contain numerous Su(var)3-9 homologues (SUVH), the members of which form a discrete subfamily. The SU(VAR)3-9 and SUVH proteins associate with heterochromatin and possess histone methyltransferase activity, indicating that they participate in the organization of transcriptionally repressive chromatin. The Arabidopsis thaliana genome contains 10 SUVH genes, belonging to four phylogenetically distinct subgroups: SUVH1, SUVH2, SUVH4, and SUVH5. The structure and expression of SUVH3, a member of the SUVH1 subgroup was investigated. SUVH3 was shown to be broadly expressed during plant development with the highest levels found in proliferating cells. The encoded protein localized in subnuclear foci and remained associated with condensed chromosomes throughout mitosis. A deletion analysis of the SUVH3 upstream region further revealed that an intron located in the 5' UTR is a key regulator of strong and constitutive SUVH3 expression.
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http://dx.doi.org/10.1093/jxb/erl093 | DOI Listing |
J Integr Plant Biol
February 2019
National Institute of Biological Sciences, Beijing 102206, China.
Although the mechanism of DNA methylation-mediated gene silencing is extensively studied, relatively little is known about how promoter methylated genes are protected from transcriptional silencing. SUVH1, an Arabidopsis Su(var)3-9 homolog, was previously shown to be required for the expression of a few promoter methylated genes. By chromatin immunoprecipitation combined with sequencing, we demonstrate that SUVH1 binds to methylated genomic loci targeted by RNA-directed DNA methylation.
View Article and Find Full Text PDFScience
December 2018
Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
DNA methylation generally functions as a repressive transcriptional signal, but it is also known to activate gene expression. In either case, the downstream factors remain largely unknown. By using comparative interactomics, we isolated proteins in that associate with methylated DNA.
View Article and Find Full Text PDFJ Exp Bot
December 2006
Smurfit Institute of Genetics, Trinity College, Dublin 2, Ireland.
SU(VAR)3-9 proteins are key regulators of heterochromatin structure and function in plants, mammals, Drosophila, and yeast. In contrast to animals and fungi, plants contain numerous Su(var)3-9 homologues (SUVH), the members of which form a discrete subfamily. The SU(VAR)3-9 and SUVH proteins associate with heterochromatin and possess histone methyltransferase activity, indicating that they participate in the organization of transcriptionally repressive chromatin.
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