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Authenticity and Bioactive Markers Search in the Phenolic-Rich Extracts of Asteraceae Medicinal Plants Through Integrative Computational Chemometrics.

Food Sci Nutr

January 2025

Nutrition and Bromatology Group, Department of Analytical Chemistry and Food Science, Instituto de Agroecoloxía e Alimentación (IAA) - CITEXVI Universidade de Vigo Vigo Spain.

The Asteraceae family has been of significant concern for ethnobotanical studies, thanks to its health-promoting properties linked to a plethora of bioactive compounds, among which phenolic compounds play a critical role. In this work, a workflow based on computational chemometrics was employed to assess the authenticity and biomarker search of five key Asteraceae species commonly employed in traditional medicine. The UHPLC-DAD-ESI/MS-MS phenolic profile of Asteraceae extracts was combined with the evaluation of several in vitro biological properties.

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Platelet-derived growth factor alpha (PDGFRA) plays a significant role in various malignant tumors. PDGFRA expression boosts thyroid cancer cell proliferation and metastasis. Radiorefractory thyroid cancer is poorly differentiated, very aggressive, and resistant to radioiodine therapy.

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Members of the genus are the conventional medicinal plants used in the therapeutic management of numerous ailments, especially for their antioxidant and pharmacological activities. The crude extract of was profiled using high-resolution GC-MS and LC-MS/MS techniques to determine possible bioactive compounds that are vital to the antioxidant activity. A total of 52 and 63 bioactive compounds have been detected in GC-MS chromatograms using different solvents (methanol and ethanol) in leaf extracts, representing the presence of certain bioactive compounds.

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Background: The German cockroach () is a pest with a global distribution that has adapted to live in human environments. threatens human health by producing asthma-inducing allergens, carrying pathogenic/antibiotic-resistant microbes, and contributing to unhealthy indoor environments. Effective application of insecticides can play an important role in cockroach control programs.

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As an efficient gene editing tool, the CRISPR/Cas9 system has been widely employed to investigate and regulate the biosynthetic pathways of active ingredients in medicinal plants. CRISPR technology holds significant potential for enhancing both the yield and quality of active ingredients in medicinal plants. By precisely regulating the expression of key enzymes and transcription factors, CRISPR technology not only deepens our understanding of secondary metabolic pathways in medicinal plants but also opens new avenues for drug development and the modernization of traditional Chinese medicine.

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