The technics of supercritical carbon dioxide extraction of Brucea Javanica for injection oil was studied. The effects of the conditions of extraction and separation on extraction oil yield and quality were investigated and the effects of extraction pressure and separation pressure on acid number of the oil were discussed. So the optimal condition of this method was determined. With a quality evaluation, the Brucea Javanica oil extracted with supercritical CO2 agreed with the quality standard of refined Brucea Javanica oil for injection. This method shows sufficiently the advantages of supercritical CO2 extraction applied in the extraction of traditional Chinese medicine.

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