Objective: To investigate the effect of vasoactive intestinal polypeptide (VIP) on differentiation of hematopoietic stem cells (HSC) into hepatic related cells and probe into the possibility that VIP affects HSC transdifferentiation.
Methods: Mini MACS assay was used to purify human CD34+ cells from mononuclear cell (MNC), the purity of the CD34+ cells was evaluated by flow cytometry. Alpha fetal proteins(AFP) in cultured CD34+ cells and their supernatant were measured with ELISA assay. Liver tissue markers on CD34+ cells, AFP, albumin (ALB) and CK-19, were measured by immunohistochemistry. Western blot assay was used to detect the expression of ALB on CD34+ cells. Nest RT-PCR was used to measure the expression of AFP mRNA and ALB mRNA on CD34+ cells, the product of ALB was chosen to measure the sequence.
Results: Immunohistochemistry showed that human CD34+ cell contained AFP and albumin but no CK-19 protein. When human CD34+ cells were cultured with VIP for 14 days, the concentration of AFP within CD34+ cells was decreased from (270.00 +/- 11.37) pg/mL to (165.00 +/- 8.51) pg/mL (P < 0.05). Western blot revealed that albumin in CD34+ cells treated with VIP faded, compared to that of control. Both human cord blood MNC and CD34+ cells expressed AFP mRNA and albumin mRNA; the sequence of the product of nest RT-PCR for albumin matched completely with the sequence of albumin in GenBank.
Conclusion: Human cord blood CD34+ cells have been note to express hepatocyte related markers such as AFP and albumin, although there is no evidence of CK-19 expression. These findings suggests the possibility that human HSC could be transdifferentiated into hepatocyte. The expressions of AFP and albumin on human CD34+ cells depressed by VIP indicate that VIP might inhibit the transdifferentiation of HSC.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Robust differentiation and potent immunomodulation of human mesenchymal stromal cells cultured with a xeno-free GMP protein supplement.
Cytotherapy
January 2025
Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Barcelona, Spain. Electronic address:
Background/aims: Human mesenchymal stromal cells (hMSC) are multipotent adult cells commonly used in regenerative medicine as advanced therapy medicinal products. The expansion of these cells in xeno-free supplements is highly encouraged by regulatory agencies due to safety concerns. However, the number of supplements with robust performance and consistency for hMSC expansion are limited.
View Article and Find Full Text PDFDermatofibrosarcoma protuberans arising in the lower labial mucosa: a case report and literature review.
Int J Oral Maxillofac Surg
January 2025
Department of Oral and Maxillofacial Surgery, Tsukuba Gakuen Hospital, Tsukuba, Ibaraki, Japan.
Dermatofibrosarcoma protuberans (DFSP) is a low-grade, malignant, spindle cell tumour with an infiltrative growth pattern and a high local recurrence rate. Cases of oral DFSP are rare. This report describes a case of DFSP occurring in the labial mucosa.
View Article and Find Full Text PDFComparison of Vessel Responses Following Combined Sirolimus-Eluting and Endothelial Progenitor Cell Stent and Ultra-Thin Sirolimus-Eluting Stent Implantation by Serial Optical Coherence Tomography and Coronary Angioscopy: A Multicenter Observational Study.
Am J Cardiol
January 2025
Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, Suita, Japan.
A dual-therapy sirolimus-eluting and CD34+ antibody-coated Combo Stent (DTS) has been developed to enhance endothelization and capture endothelial progenitor cells; however, vessel responses following DTS implantation remain unclear. Therefore, we evaluated early- and mid-term intravascular characteristics of DTS using intravascular imaging modalities. This multicenter, prospective, observational study enrolled 88 patients (95 lesions) who underwent DTS (43 patients, 48 lesions) or sirolimus-eluting Orsiro stent (SES, 45 patients, 47 lesions) implantation.
View Article and Find Full Text PDFBlack Chokeberry () Juice Supplementation Affects Age-Related Myocardial Remodeling in Rats.
Life (Basel)
December 2024
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Laboratory of Biologically Active Substances, 4000 Plovdiv, Bulgaria.
Background: Cardiac aging is associated with myocardial remodeling and reduced angiogenesis. Counteracting these changes with natural products is a preventive strategy with great potential. The aim of this study was to evaluate the effect of fruit juice (AMJ) supplementation on age-related myocardial remodeling in aged rat hearts.
View Article and Find Full Text PDFA Senescent Cluster in Aged Human Hematopoietic Stem Cell Compartment as Target for Senotherapy.
Int J Mol Sci
January 2025
Department of Medicine V, Heidelberg University, 69117 Heidelberg, Germany.
To identify the differences between aged and young human hematopoiesis, we performed a direct comparison of aged and young human hematopoietic stem and progenitor cells (HSPCs). Alterations in transcriptome profiles upon aging between humans and mice were then compared. Human specimens consist of CD34+ cells from bone marrow, and mouse specimens of hematopoietic stem cells (HSCs; Lin- Kit+ Sca1+ CD150+).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!