A 2918 bp sequence coding for the orotidine-5'-monophosphate decarboxylase enzyme (OMPD) was isolated from the genome of Myrothecium gramineum. This sequence was analysed and, remarkably, it is the first OMPD gene of a Sordariomycete that has an intron. The gene codes for an enzyme of 282 amino acids. The nucleotide sequence and the amino acid sequence were compared with fungal OMPD sequences. They show the highest similarity to OMPD genes and enzymes of Aspergillus sp., Penicillium sp. and Cladosporium fulvum. The functionality of the gene as a selection marker was proven by complementation of the uracil auxotrophy of Aspergillus nidulans FGSC A722.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1111/j.1574-6968.2006.00373.x | DOI Listing |
Acta Pharm Sin B
September 2023
Guangdong Provincial Key Laboratory of Tumor Interventional Diagnosis and Treatment, Zhuhai Institute of Translational Medicine, Zhuhai People's Hospital Affiliated with Jinan University, Jinan University, Zhuhai 519000, China.
Interleukin (IL)-17A, a pro-inflammatory cytokine, is a fundamental function in the onset and advancement of multiple immune diseases. To uncover the primary compounds with IL-17A inhibitory activity, a large-scale screening of the library of traditional Chinese medicine constituents and microbial secondary metabolites was conducted using splenic cells from IL-17A-GFP reporter mice cultured under Th17-priming conditions. Our results indicated that some aureane-type sesquiterpene tetraketides isolated from a wetland mud-derived fungus, , showed remarkable IL-17A inhibitory activity.
View Article and Find Full Text PDFMed Mycol Case Rep
September 2019
Department of Ocular Microbiology, Aravind Eye Hospital, No.1, Anna Nagar, Madurai, 625 020, Tamil Nadu, India.
We report two cases of keratitis caused by a rare fungus species. Clinical presentation, identification, and management were studied. Both fungi were grown from corneal scraping and identified as and based on the microbiological techniques and DNA sequencing analysis.
View Article and Find Full Text PDFAppl Microbiol Biotechnol
October 2017
Geomicrobiology Group, School of Life Sciences, University of Dundee, Dundee, , DD1 5EH, Scotland, UK.
In this research, the ureolytic fungi Neurospora crassa, Pestalotiopsis sp. and Myrothecium gramineum were investigated for the preparation of nanoscale copper carbonate and the role of fungal extracellular protein in such mineral formation. After incubation in urea-modified media, carbonate-laden fungal supernatants were used for the precipitation of copper carbonate, with experimental results agreeing closely with those obtained using geochemical modelling (Geochemist's Workbench).
View Article and Find Full Text PDFCarbohydr Polym
May 2016
Analytical Division, CSIR-North East Institute of Science and Technology, Jorhat 785 006, Assam, India.
Cellulose was extracted from agricultural waste like Rice Husk (RH) a renewable resource of India as well as in the World. Cellulose was isolated from rice husk (RH) using eco-friendly method with Montmorillonite K-10/LiOH solution and bleaching with 2% H2O2. The reaction parameters like time, temperature, catalyst, acid and alkali were studied to evaluate the optimum reaction conditions 6h, 80°C, 20% maleic acid and 10% LiOH (in H2O) for time, temperature, acid and alkali, respectively.
View Article and Find Full Text PDFEnviron Microbiol
August 2015
Geomicrobiology Group, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, Scotland, UK.
The urease-positive fungi Pestalotiopsis sp. and Myrothecium gramineum, isolated from calcareous soil, were examined for their properties of CaCO3 and SrCO3 biomineralization. After incubation in media amended with urea and CaCl2 and/or SrCl2 , calcite (CaCO3 ), strontianite (SrCO3 ), vaterite in different forms [CaCO3 , (Cax Sr1-x )CO3 ] and olekminskite [Sr(Sr,Ca)(CO3 )2 ] were precipitated, and fungal 'footprints' were observed on mineral surfaces.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!