We developed a capillary zone electrophoresis method with indirect UV detection for determination of ammonium cations and alkali and alkaline earth metal cations in jellyfish. As the background electrolyte, a mixture of N-methylbenzylamine, citrate, and 18-crown-6 was used for the complete separation of all analyte cations. The limits of detection were 0.13 - 0.34 mg l(-1) at a signal-to-noise ratio of three. The values of the relative standard deviation of peak area were 3.2 - 4.9%. The proposed method successfully determined the above analyte cations in jellyfish for approximately 4 min.
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http://dx.doi.org/10.2116/analsci.22.1129 | DOI Listing |
Int J Mol Sci
April 2023
Photobiology Laboratory, Institute of Biophysics of Siberian Branch of the Russian Academy of Sciences, Federal Research Center "Krasnoyarsk Science Center" of Siberian Branch of the Russian Academy of Sciences", Krasnoyarsk 660036, Russia.
Hydromedusan photoproteins responsible for the bioluminescence of a variety of marine jellyfish and hydroids are a unique biochemical system recognized as a stable enzyme-substrate complex consisting of apoprotein and preoxygenated coelenterazine, which is tightly bound in the protein inner cavity. The binding of calcium ions to the photoprotein molecule is only required to initiate the light emission reaction. Although numerous experimental and theoretical studies on the bioluminescence of these photoproteins were performed, many features of their functioning are yet unclear.
View Article and Find Full Text PDFInt J Biol Macromol
March 2023
Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), No. 1 Wenhai Road, Qingdao 266237, China. Electronic address:
Jellyfish Cyanea nozakii venom is a complex mixture of various toxins, most of which are proteinous biological macromolecules and are considered to be responsible for clinical symptoms or even death after a severe sting. Previous transcriptome and proteome analysis identified hundreds of toxins in the venom, including hemolysins, C-type lectin, phospholipase A, potassium channel inhibitor, metalloprotease, etc. However, it is not clear which toxin in the venom plays the most important role in lethality.
View Article and Find Full Text PDFCommun Biol
January 2023
Department of Ecology, Evolution and Behavior, Alexander Silberman Institute of Life Sciences, Faculty of Science, The Hebrew University of Jerusalem, Jerusalem, Israel.
Ion channels of the DEG/ENaC family share a similar structure but serve strikingly diverse biological functions, such as Na reabsorption, mechanosensing, proton-sensing, chemosensing and cell-cell communication via neuropeptides. This functional diversity raises the question of the ancient function of DEG/ENaCs. Using an extensive phylogenetic analysis across many different animal groups, we found a surprising diversity of DEG/ENaCs already in Cnidaria (corals, sea anemones, hydroids and jellyfish).
View Article and Find Full Text PDFNeurotox Res
December 2020
Department of Physiology, School of Medicine, Jeju National University, Jeju, 63243, Republic of Korea.
Int J Parasitol
September 2020
Department of Microbiology, Oregon State University, Corvallis, OR, USA. Electronic address:
Myxozoans are obligate parasites that have complex life cycles requiring alternate vertebrate and invertebrate hosts, with transmission via microscopic waterborne spores. Unusually for parasites, they belong to the phylum Cnidaria, alongside thousands of free-living corals, sea anemones, jellyfish and hydrozoans. Their cnidarian affinity is affirmed by genetic relatedness and the presence of nematocysts, historically called "polar capsules" in myxozoan research.
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