A novel double-antigen sandwich enzyme-linked immunosorbent assay for Measurement of Antibodies against rabies virus.

Clin Vaccine Immunol

The Center for Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Zhongguancun Beijing, China.

Published: August 2006

A novel double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) was developed to measure rabies antibodies in dogs. In contrast to the 4 days required for detecting rabies antibody with conventional rabies antibody virus neutralization assays, this ELISA can be completed in hours, without using live virus, in routine laboratories.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1539129PMC
http://dx.doi.org/10.1128/CVI.00102-06DOI Listing

Publication Analysis

Top Keywords

novel double-antigen
8
double-antigen sandwich
8
sandwich enzyme-linked
8
enzyme-linked immunosorbent
8
immunosorbent assay
8
rabies antibody
8
assay measurement
4
measurement antibodies
4
rabies
4
antibodies rabies
4

Similar Publications

Crimean-Congo haemorrhagic fever orthonairovirus (CCHFV) and Dugbe orthonairovirus (DUGV) are zoonotic viruses transmitted by ticks. Whereas CCHFV has caused numerous human cases, DUGV, although less reported, shares ticks and ruminants as hosts. Since its first discovery in Nigeria in 1964, there has been no detailed sero-epidemiological investigation on DUGV in sub-Saharan Africa.

View Article and Find Full Text PDF

Development of a fully automated chemiluminescent immunoassay for the quantitative and qualitative detection of antibodies against African swine fever virus p72.

Microbiol Spectr

October 2024

College of Animal Science, National Engineering Center for Swine Breeding Industry, State Key Laboratory of Swine and Poultry Breeding Industry, South China Agricultural University, Guangzhou, China.

African swine fever (ASF), caused by ASF virus (ASFV), is a highly infectious and severe hemorrhagic disease of pigs that causes major economic losses. Currently, no commercial vaccine is available and prevention and control of ASF relies mainly on early diagnosis. Here, a novel automated double antigen sandwich chemiluminescent immunoassay (DAgS-aCLIA) was developed to detect antibodies against ASFV p72 (p72-Ab).

View Article and Find Full Text PDF

Rapid and reliable detection of Leishmania antibodies in canine serum with double-antigen sandwich homogeneous chemical luminescence.

Parasit Vectors

July 2024

National Key Laboratory of Veterinary Public Health Safety, Beijing Key Laboratory of Detection Technology for Animal Derived Food Safety, Beijing Laboratory for Food Quality and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People's Republic of China.

Background: Leishmaniasis, caused by Leishmania spp. parasites, is an important zoonotic disease globally, posing severe threats to humans and animals. In the absence of effective vaccines, reliable serological diagnostic methods are critical for disease control.

View Article and Find Full Text PDF

A novel quantitative double antigen sandwich ELISA for detecting total antibodies against Candida albicans enolase 1.

Eur J Clin Microbiol Infect Dis

September 2024

Basic Medicine Laboratory, Bethune International Peace Hospital, No.398 West Zhongshan Road, Shijiazhuang, Hebei, 050082, P.R. China.

Purpose: This study aimed to develop a double antigen sandwich ELISA (DAgS-ELISA) method for more efficient, accurate, and quantitative detection of total antibodies against Candida albicans enolase1 (CaEno1) for diagnosing invasive candidiasis (IC).

Methods: DAgS-ELISA was developed using recombinant CaEno1 and a monoclonal antibody as the standard. Performance evaluation included limit of detection, accuracy, and repeatability.

View Article and Find Full Text PDF

African swine fever (ASF), caused by ASF virus (ASFV), is a highly contagious and lethal disease of domestic pigs leading to tremendous economic losses. As there are no vaccines and drugs available. An effective diagnosis to eliminate ASFV-infected pigs is a crucial strategy to prevent and control ASF.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!