Fructo-oligosaccharides (FOS) represent the most abundantly supplied and utilized group of nondigestible oligosaccharides as food ingredients. These prebiotics can be produced from sucrose using the transglycosylating activity of beta-fructofuranosidases (EC 3.2.1.26) at high concentrations of the starting material. The main problem during FOS synthesis is that the activity of the enzyme is inhibited by the glucose generated during the reaction, and therefore the maximum FOS content in commercial products reaches up to 60% on a dry substance basis. The glucose oxidase (gox) gene from Aspergillus niger BT18 was cloned and integrated, as part of an expression cassette, into the ribosomal DNA of a Saccharomyces cerevisiae host strain. One of the recombinant strains with a high copy number of the gox gene and showing a high GOX specific activity was used to produce the enzyme. Addition of the extracellular glucose oxidase to the FOS synthesis reaction helped to remove the glucose generated, avoiding the inhibition of the fungal beta-fructofuranosidase. As a result, a final syrup containing up to 90% of FOS was obtained.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/bp060076k | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A pH/GSH Dual-Responsive Triple Synergistic Bimetallic Nanocatalyst for Enhanced Tumor Chemodynamic Therapy.
Small
January 2025
Department of Thyroid Surgery, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710000, China.
Chemodynamic therapy (CDT) has garnered significant attention in the field of tumor therapy due to its ability to convert overexpressed hydrogen peroxide (HO) in tumors into highly toxic hydroxyl radicals (•OH) through metal ion-mediated catalysis. However, the effectiveness of CDT is hindered by low catalyst efficiency, insufficient intra-tumor HO level, and excessive glutathione (GSH). In this study, a pH/GSH dual responsive bimetallic nanocatalytic system (CuFeMOF@GOx@Mem) is developed by modifying red blood cell membranes onto glucose oxidase (GOx)-loaded Fe-Cu bimetallic MOFs, enhancing the efficacy of CDT through a triple-enhanced way by HO self-supply, catalysts self-cycling, and GSH self-elimination.
View Article and Find Full Text PDFA Molecularly Imprinted Electrochemical Sensor for Carbendazim Detection Based on Synergy Amplified Effect of Bioelectrocatalysis and Nanocomposites.
Polymers (Basel)
January 2025
Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, China.
The highly selective and sensitive determination of pesticide residues in food is critical for human health protection. Herein, the specific selectivity of molecularly imprinted polymers (MIPs) was proposed to construct an electrochemical sensor for the detection of carbendazim (CBD), one of the famous broad-spectrum fungicides, by combining with the synergistic effect of bioelectrocatalysis and nanocomposites. Gold nanoparticle-reduced graphene oxide (AuNP-rGO) composites were electrodeposited on a polished glassy carbon electrode (GCE).
View Article and Find Full Text PDFFabrication of Compartmentalized Multienzyme Reactor for Colorimetric Biosensing of Glucose and Phenol with High Sensitivity.
ACS Appl Mater Interfaces
January 2025
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, P. R. China.
Enzymatic cascade reactions are widely utilized in food security, environmental monitoring, and disease diagnostics, whereas their practical application was hindered due to their limited catalytic efficiency and intrinsic fragility to environmental influences. Herein, a compartmentalized dual-enzyme cascade nanoreactor was constructed in metal-organic frameworks (ZIF-8) by a shell-by-shell growth method. ZIF-8 provided a good microenvironment to maintain the activity of enzymes and protected them against harsh conditions.
View Article and Find Full Text PDFRational design of redox active metal organic frameworks for mediated electron transfer of enzymes.
Mater Horiz
January 2025
Department of Material Sciences, Institute of Pure and Applied Sciences, University of Tsukuba, 1-1-1, Tennodai, Ibaraki 305-5358, Japan.
The efficient immobilization of redox mediators remains a major challenge in the design of mediated enzyme electrode platforms. In addition to stability, the ability of the redox-active material to mediate electron transfer from the active-site buried enzymes, such as flavin adenine dinucleotide-dependent glucose dehydrogenase (FADGDH) and lactate oxidase (LOx), is also crucial. Conventional immobilization techniques can be synthetically challenging, and immobilized mediators often exhibit limited durability, particularly in continuous operation.
View Article and Find Full Text PDFSelf-Cascade of ROS/Glucose-Scavenging Immunomodulatory Hydrogels for Programmed Therapeutics of Infected Diabetic Ulcers via Nrf2/NF-κB Pathway.
Small
January 2025
Guangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, and Guangdong-Hong Kong-Macao Joint Laboratory for New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, Guangdong, 510515, China.
Diabetic ulcers (DUs) are characterized by a microenvironment with high oxidative stress, high blood glucose levels, and recalcitrant bacterial infections. This microenvironment is accompanied by long-term suppression of endogenous antioxidant systems, which makes their clinical management extremely challenging. To address this issue, a hybridized novel gold-palladium (AuPd) nanoshell of the injectable/injectable hydrogel system UiO/AuPd/BNN6/PEG@Gel (UAPsBP@Gel) is developed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!