Objective: To study the feasibility of application of rat-tail collagen in the primary culture of rats marginal cells of stria vascularis.
Method: The effect of self-made rat-tail collagen on the primary culture of rats marginal cells of stria vascularis was observed and estimated.
Result: When cochlear stria vascularis fragment was isolated and cultured for 24 h, a few culture cells appeared around the fragments. About 48 to approximately 72 h later, clusters of culture cells could be seen,these cells showed the cobblestone shape under the microscope. The immunohistochemistry and the transmission electron microscopy showed the epithelial origination of these cells.
Conclusion: It is feasible to the primarily culture the rats marginal cells of stria vascularis with self-made rat-tail collagen.
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