Crystallization and initial X-ray analysis of polyhydroxyalkanoate granule-associated protein from Aeromonas hydrophila.

Acta Crystallogr Sect F Struct Biol Cryst Commun

MOE Key Laboratory of Protein Science, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China.

Published: August 2006

Polyhydroxyalkanoate (PHA) granule-associated proteins (phasins) were discovered in PHA-accumulating bacteria. They play a crucial role as a structural protein during initial PHA-granule formation and granule growth and also serve as interfaces for granule stabilization in vivo. The phasin PhaP(Ah) from Aeromonas hydrophila strain 4AK4 was crystallized using the hanging-drop vapour-diffusion method. Single crystals were cryocooled for X-ray diffraction analysis. The phasin crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 80.8, b = 108.9, c = 134.4 angstroms.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242923PMC
http://dx.doi.org/10.1107/S1744309106025000DOI Listing

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