AI Article Synopsis
- A fragment of the prohibitin (Phb) gene was isolated and its expression in wild-type and mutant Arabidopsis thaliana seeds was analyzed, revealing high abundance of Phb mRNA in wild-type seeds.
- The 3' untranslated region of the Phb gene exhibits notable features that may contribute to mRNA stability.
- The study found that Phb in Arabidopsis does not interact with certain proteins (retinoblastoma-related protein or E2F) as seen in mammals, suggesting a different role in cell cycle regulation during seed germination.
Article Abstract
A fragment encoding a partial sequence of a prohibitin (Phb) gene was isolated. The expression of Phb mRNA and protein in seeds of wild type and mutant Arabidopsis thaliana is presented. Phb mRNA is abundant in wild-type seeds; thus, it may have sequence or structural characteristics responsible for this stability. The 3' untranslated region sequence of a Phb gene has interesting features. We found that Arabidopsis Phb does not interact with a retinoblastoma-related protein or E2F in a yeast two-hybrid system, thus suggesting that the plant protein may have not conserved such interaction, described for mammalian Phb. The possible role of Phb in cell cycle regulation during germination is discussed.
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| http://dx.doi.org/10.1016/j.jplph.2006.05.002 | DOI Listing |
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