AI Article Synopsis
- Scientists found that a special non-coding RNA called Air helps silence certain genes in a specific part of DNA!
- Air is made using a special process, but it doesn't get cut into smaller pieces like most RNA does, making it super long and unstable!
- This study shows that Air's ability to silence genes might not be just because of the RNA itself, but more about how it is made in the cell!
Article Abstract
Expression of the Air ncRNA is necessary to silence multiple genes in cis in the imprinted Igf2r cluster. However, its mode of action is unknown. Here, we characterize co- and post-transcriptional features of Air that identify it as a new member of the class of nuclear regulatory RNAs. We show that Air is transcribed from a DNA methylation-sensitive promoter by RNA polymerase II (RNAPII). However, although it is capped and polyadenylated similar to other RNAPII transcripts, the majority of Air transcripts evade cotranscriptional splicing resulting in a mature 108 kb ncRNA. As a consequence, the mature unspliced Air is nuclear localized and highly unstable. These features show that Air is an atypical RNAPII transcript whose properties indicate that its mode of action in gene silencing may not depend on the RNA per se but instead is related to its actual transcription.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1538572 | PMC |
| http://dx.doi.org/10.1038/sj.emboj.7601245 | DOI Listing |
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