AI Article Synopsis
- siRNAs and shRNAs, typically 21 base pairs long, are used for RNA interference, but longer variants have been found to enhance gene silencing potency.
- A new technique was developed to create shRNA libraries with varying stem lengths from firefly luciferase and Jurkat cDNA, allowing for more effective gene silencing.
- This method enables the rapid and cost-effective preparation of shRNA libraries across species, facilitating gene function studies and potential drug discovery targets.
Article Abstract
Short interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs) usually used for RNA interference (RNAi) are double-stranded RNAs (dsRNAs) of 21 base pairs. However, siRNAs and shRNAs of longer stem length have been reported to show more potent gene silencing. Here, we report a new technique to enzymatically construct shRNA libraries containing clones from firefly luciferase cDNA and Jurkat cDNA. The technique allowed the efficacious generation of shRNAs of arbitrary stem length as desired, providing the clones which potently silenced the specified gene expression and presenting a high efficiency rate of gene silencing. Our results indicate that the technique permits the rapid, efficient, and low-cost preparation of genomewide shRNA expression libraries not only for humans and mice but also for sorts of biological species and that the relevant libraries are applicable for the search of genes related to phenotype changes and of new targets for drug discovery.
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| http://dx.doi.org/10.1016/j.bbrc.2006.05.124 | DOI Listing |
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