AI Article Synopsis
- The study aimed to explore the effects of curcumin (Cur) on colitis induced by TNBS and its influence on the balance of Th1/Th2 cytokines.
- The methods involved administering Cur and dexamethasone (Dex) to assess their impact on body weight, histological changes, and cytokine expression in colon tissues.
- Results showed that Cur improved colitis symptoms, reduced Th1 cytokine levels, and promoted Th2 cytokine levels, whereas Dex didn't have the same effect on Th2 cytokines.*
Article Abstract
Aim: To investigate the therapeutic effects of curcumin (Cur) on trinitrobenzene sulphonic acid (TNBS)-induced colitis and the effects of Cur on the balance of Th1/Th2 cytokines.
Methods: Colitis was induced by TNBS and treated with Cur (30 mg/kg/d, ip), dexamethasone (Dex, 2 mg/kg/d), or Cur plus dexamethasone (Cur+Dex, 30 mg/kg/d Cur ip+2 mg/kg/d Dex,ip). mRNA in colon mucosa were detected by real-time quantitative polymerase chain reaction. Intracellular cytokines were detected by flow cytometry and concentrations of cytokines in sera were detected by enzyme-linked immunosorbent analysis.
Results: Both Cur and Dex improved body weight loss, ameliorated histological images and decreased macroscopic score and myeloperoxidase activity. Cur decreased the expression of Th1 cytokines (IL-12, IFN-gamma, TNF-alpha, IL-1) and increased the expression of Th2 cytokines (IL-4 and IL-10) in colon mucosa. Cur also increased the proportion of IFN-gamma/IL-4 in splenocytes and circulation. Dex and Cur+Dex decreased the expression of Th1 cytokines but could not increase the expression of Th2 cytokines and the proportion of IFN-gamma/IL-4.
Conclusion: Cur exerted therapeutic effects on colitis by regulating the shift from Th1 to Th2.
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| http://dx.doi.org/10.1111/j.1745-7254.2006.00322.x | DOI Listing |
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