Inelastic neutron scattering studies of the interaction between water and some amino acids.

The interaction between water and some of amino acids (glycine, L-glutamine, L-threonine, L-cysteine and L-serine) was studied by inelastic incoherent neutron scattering (IINS). The vibrational spectra of dry amino acids and amino acids with a water content (e.g., 1 mol water/1 mol amino acid) were recorded. Comparing the difference spectra obtained by subtracting the spectrum of dry sample from those of wet sample with the spectra of ice Ih, we obtained that the difference spectrum for serine changed greatly from normal ice spectrum; but on the other hand, the difference spectra for the other amino acids such as glycine, glutamine, threonine, and cysteine changed slightly. The results demonstrate that serine has stronger hydrophilic character than glycine, glutamine, threonine, and cysteine. This is the first time the hydrophilic or hydrophobic character of amino acids was studied by using inelastic neutron scattering techniques, which provides important information for theoretical modeling and force field refinement for the interaction between water and the amino acids studied here.