AI Article Synopsis
- The study examines how phosvitin affects the formation of hydroxyapatite (HAP) and its role in preventing the conversion of amorphous calcium phosphate (ACP) to HAP.
- Phosvitin significantly boosts the nucleation rate of HAP on a collagen surface, allowing for uniform crystal growth compared to the slow, uneven nucleation observed without phosvitin.
- Additionally, the presence of free phosvitin hinders the transformation from ACP to HAP, leading to a shift in the growth process instead of a direct transformation.
Article Abstract
The promoting effect of phosvitin on the nucleation of hydroxyapatite (HAP) and the inhibitory effect of phosvitin on the transformation from amorphous calcium phosphate (ACP) to HAP were investigated. Atomic force microscopy observations showed that the nucleation of HAP on collagen substrate was greatly enhanced when the phosvitin was bound on the collagen surface. Nucleated crystals were uniformly distributed with a high nucleation rate on the collagen surface in the presence of phosvitin, while, in the absence of phosvitin, crystals nucleated slowly and were observed only at some particular area. Time-resolved static light scattering measurements revealed that the transformation from ACP to HAP was inhibited when free phosvitin was present in the calcium phosphate solutions. The transformation kinetics in the absence of phosvitin, which is a direct reconstruction of the inner ACP structure to HAP, was changed to heterogeneous growth of HAP on ACP with time.
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| http://dx.doi.org/10.1021/jp044550l | DOI Listing |
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