Objective: To identify sarcosaphagous flies and their larvae, pupa.

Methods: Sarcosaphagous flies and their larvae, pupas were collected from human corpses and their surroundings in the Weifang city. A 304 bp region in COI gene was analyzed by mtDNA sequencing.

Results: The studied region showed no sequence divergence within same species and significant difference were found between different species in all samples.

Conclusion: It is a practical approach to identify these Sarcosaphagous flies and their larvae, pupas by sequence analysis of the 304bp region of the COI in mtDNA.

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