The Na+/H+ exchangers (NHEs) catalyze the transport of Na+ in exchange for H+ across membranes in organisms and are required for numerous physiological processes. Here we report the cloning and characterization of a novel human NHEDC1 (Na+/H+ exchanger like domain containing 1) gene, which was mapped to human chromosome 4p24. This cDNA is 1859 bp in length, encoding a putative protein of 515 amino acids. The NHEDC1 proteins are highly conserved in mammals including human, mouse, rat, and Macaca fascicularis. One remarkable characteristic of human NHEDC1 gene is that it is exclusively expressed in the testis by RT-PCR analysis. Western blot analysis showed that the molecular weight of NHEDC1 is about 56 KDa.
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SLC9B1 methylation predicts fetal intolerance of labor.
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a Genetics and Molecular Biology Program , Emory University, 1462 Clifton Road, Atlanta , GA , 30322.
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- Fetal intolerance of labor, indicated by abnormal fetal heart rate patterns, often leads to Caesarean deliveries and is linked to fetal distress like hypoxia and metabolic acidemia.
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Identification and characterization of methylation-dependent/independent DNA regulatory elements in the human SLC9B1 gene.
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Department of Biology, Miami University, Oxford, OH, United States. Electronic address:
The human NHEDC1 (hNHEDC1) protein is thought to be essential for sperm motility and fertility however the mechanisms regulating its gene expression are largely unknown. In this study we have identified multiple DNA regulatory elements in the 5' end of the gene encoding hNHEDC1 (SLC9B1) and have explored the role that DNA methylation at these elements plays in the regulation of its expression. We first show that the full-length hNHEDC1 protein is testis-specific for the tissues that we tested and that it localizes to the cells of the seminiferous tubules.
View Article and Find Full Text PDFCloning of a novel human NHEDC1 (Na+/H+ exchanger like domain containing 1) gene expressed specifically in testis.
Mol Biol Rep
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Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.
The Na+/H+ exchangers (NHEs) catalyze the transport of Na+ in exchange for H+ across membranes in organisms and are required for numerous physiological processes. Here we report the cloning and characterization of a novel human NHEDC1 (Na+/H+ exchanger like domain containing 1) gene, which was mapped to human chromosome 4p24. This cDNA is 1859 bp in length, encoding a putative protein of 515 amino acids.
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