AI Article Synopsis
- The study investigates the role of apoptosis in regulating olfactory receptor neuron populations, shedding light on neurogenesis.
- Using TUNEL and electron microscopy, the researchers analyzed apoptosis in the olfactory mucosa of both healthy and bulbectomized rats over varying time periods.
- Findings suggest that apoptosis contributes to the turnover and regeneration of the olfactory epithelium, with evidence for additional types of neural death occurring through distinct mechanisms.
Article Abstract
Objective: To study whether apoptosis plays a role in controlling the number of olfactory receptor neurons, so as to reveal the specialty and mystery of neurogenesis.
Methods: Using terminal deoxynucleotidyl transferase-mediated dUTP-fluorescein nick end labeling (TUNEL) and transmission electron microscopy to detect apoptosis in olfactory mucosa of normal adult rats and damaged olfactory mucosa of 16, 32, 48 hours and 3, 7, 30 days after bulbectomy.
Results: In normal olfactory epithelium, a subpopulation of immature neurons, as well as mature neurons, showed internucleosomal DNA-fragmentation. The number of TUNEL-labeled neurons increased dramatically 32 hours after removal of olfactory bulb. Then it declined quickly and remained at low level. Ultrastructural data of olfactory mucosa showed that the feature of apoptotic neurons was chromatin condensation and cell shrinkage. Besides, some dying cells were characterized by the formation of numerous autophagic vacuoles, and few had some of the features of necrosis but without obvious mitochondrial swelling.
Conclusions: Apoptosis might play a role in turnover of the olfactory epithelium and regeneration in adult rats. There might be other two types of neural death through different mechanism.
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