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Application of aqueous two-phase extraction for separation and purification of various adeno-associated viruses.
Biotechnol Lett
January 2025
Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang Key Laboratory of Smart Biomaterials, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China.
Objective: Adeno-associated viruses (AAVs) are widely used as gene therapy vectors due to their safety, stability, and long-term expression characteristics. The objective of this work is to develop an aqueous two-phase system (ATPS) as a universal platform for the separation and purification of AAVs.
Results: This study utilized polyethylene glycol (PEG)/salt ATPSs to separate and purify various AAV serotypes, including AAV5, AAV8, and AAV9, which focusing on serotype-specific performance and partial empty capsid removal.
Thyroid Autoimmunity During Universal Salt Iodisation-Possible Short-Term Modulation with Longer-Term Stability.
Nutrients
December 2024
Thyroid Research Group, Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff CF14 4XN, UK.
Universal salt iodisation (USI) plays an essential role in the provision of iodine (I) to populations worldwide. Countries adopting USI programmes, adhering to strict criteria laid down by expert organisations such as the Iodine Global Network, are estimated to have reduced the prevalence of I deficiency by 75% (protecting 720 million individuals worldwide). Despite this success, doubts have been raised as to the desirability of continuing such programmes because of (a) the need to reduce salt intake for cardiovascular prevention and (b) the induction of thyroid autoimmunity.
View Article and Find Full Text PDFDevelopment and Validation of the Immediate Postpartum Care Adherence Questionnaire: An Application of the Theory of Planned Behavior among Healthcare Professionals.
Iran J Nurs Midwifery Res
November 2024
Nursing Department, Faculty of Nursing, Al-Balqa Applied University, Salt, Jordan.
Background: The postpartum period is critical for women and newborns. Many complications, such as deaths, are preventable by utilizing appropriate postpartum care. We aimed to assess healthcare professionals' adherence to the World Health Organization's (WHO) immediate postpartum care guidelines, investigating factors influencing their adoption at Jordanian hospitals.
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