Comparative studies were carried out on GOD-trinder method for measuring glucose in serum with four different kinds of benzene-original phenols-phenyl hydroxide, 2,4-dichlorophenol, 2,6-dichlorophenol and resorcinol. It is shown that when the three coloring systems-phenyl hydroxide, 2,4-dichlorophenol or resorcinol coupling with glucose oxidase-peroxidase-4-a minoantipyrine-hydrogen peroxide (GOD-POD-4-AA-H2O2) respectively were used for clinical assay of glucose in serum, the determination results showed no significant differences (P > 0.05). Compared with phenyl hydroxide, the coloring system of 2,4-dichlorophenol is better in the aspects of sensitivity, precision and accuracy; the resorcinol system has lower sensitivity with the advantage of faster detecting velocity, and larger linear range, and can be used for on-line rapid analysis of samples with more concentrated glucose in industry.
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