AI Article Synopsis
- FSH plays a crucial role in folliculogenesis by binding to receptors on granulosa cells, promoting communication and steroid production.
- A study using Suppression Subtractive Hybridization (SSH) on pig granulosa cells identified 25 regulated transcripts, including three novel sequences after treating with FSH.
- Gene analysis revealed diverse functions for these transcripts, such as involvement in catalytic processes, transport, signaling, binding, antioxidant activity, and structural roles, suggesting FSH's broader impact on cell function.
Article Abstract
FSH, which binds to specific receptors on granulosa cells in mammals, plays a key role in folliculogenesis. Its biological activity involves stimulation of intercellular communication and upregulation of steroidogenesis, but the entire spectrum of the genes regulated by FSH has yet to be fully characterized. In order to find new regulated transcripts, however rare, we have used a Suppression Subtractive Hybridization approach (SSH) on pig granulosa cells in primary culture treated or not with FSH. Two SSH libraries were generated and 76 clones were sequenced after selection by differential screening. Sixty four different sequences were identified, including 3 novel sequences. Experiments demonstrated the presence of 25 regulated transcripts.A gene ontology analysis of these 25 genes revealed (1) catalytic; (2) transport; (3) signal transducer; (4) binding; (5) anti-oxidant and (6) structural activities. These findings may deepen our understanding of FSH's effects. Particularly, they suggest that FSH is involved in the modulation of peroxidase activity and remodelling of chromatin.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1533831 | PMC |
| http://dx.doi.org/10.1186/1477-7827-4-35 | DOI Listing |
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