Leishmaniasis is considered by the World Health Organization to be the second most important disease caused by a protozoan parasite. Biochemical and molecular biology studies can help in the understanding of the biology of the Leishmania parasite. All protozoan parasites, including Leishmania, are unable to synthesize purines de novo, and nucleoside diphosphate kinases (NDK) are involved in the salvage pathway by which free purines are converted to nucleosides and subsequently to nucleotides. In this report, we describe the cloning of NDK coding-sequence from Leishmania major, the expression of the enzyme containing a His(6)-tag in Escherichia coli, and purification of the catalytically active native protein by affinity chromatography using Ni-NTA resin.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.pep.2006.05.010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Expression, Purification, Spectroscopic Characterization, and Membrane Topology Classification of KCNE4 from Recombinant .
J Phys Chem B
January 2025
Department of Chemistry and Biochemistry, Miami University, 651 E. High Street, Oxford, Ohio 45056, United States.
Members of the KCNE family are accessory subunits that modulate voltage-gated potassium channels. One member, KCNE4, has been shown to inhibit the potassium ion current in these channels. However, little is known about the structure, dynamics, and mode of inhibition of KCNE4, likely due to challenges in overexpressing and purifying the protein.
View Article and Find Full Text PDFGenomic characterization of Escherichia coli with a polyketide synthase (pks) island isolated from ulcerative colitis patients.
BMC Genomics
January 2025
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
The E. coli strains harboring the polyketide synthase (pks) island encode the genotoxin colibactin, a secondary metabolite reported to have severe implications for human health and for the progression of colorectal cancer. The present study involves whole-genome-wide comparison and phylogenetic analysis of pks harboring E.
View Article and Find Full Text PDFExpression and purification of Mycobacterium tuberculosis F-dependent glucose-6-phosphate dehydrogenase enzyme using Escherichia coli.
Protein Expr Purif
January 2025
Manchester Institute of Biotechnology, Department of Chemistry, University of Manchester, 131 Princess St, Manchester, M1 7DN, United Kingdom. Electronic address:
Since their discovery in Mycobacterium tuberculosis (Mtb), F-dependent enzymes have been identified as both important drug targets and potential industrial biocatalysts, including for bioremediation of otherwise recalcitrant substrates. Mtb-FGD1, utilizes glucose 6-phosphate (G6P) as an electron donor for the reduction of F. Current expression systems for Mtb-FGD1 use Mycobacterium smegmatis as host, because of the tendency for it to form inclusion bodies in E.
View Article and Find Full Text PDFEvaluation of MALDI-TOF for identification of Vibrio cholerae and Vibrio parahaemolyticus from growth on agar media.
Appl Microbiol Biotechnol
January 2025
Vibrio Reference Laboratory, Bureau of Microbial Hazards, Health Canada, Ottawa, ON, Canada.
Two methods were compared for their ability to accurately identify Vibrio species of interest: whole genome sequencing as the reference method and MALDI-TOF MS (matrix-assisted laser desorption/ionization-time of flight mass spectrometry) proteome fingerprinting. The accuracy of mass spectrometry-based identification method was evaluated for its ability to accurately identify isolates of Vibrio cholerae and Vibrio parahaemolyticus. Identification result of each isolate obtained by mass spectrometry was compared to identification by whole genome sequencing (WGS).
View Article and Find Full Text PDFLuminescent Cu nanoclusters-encapsulated ZIF-8 as on-off-on fluorescent probe for efficient and selective quantification of E. coli.
Mikrochim Acta
January 2025
Department of Physics, Punjab Engineering College (Deemed to be University), Chandigarh, 160012, India.
Rapid and accurate detection of Escherichia coli (E. coli) is critical for maintaining water quality, and protecting aquatic ecosystems and public health. This research focuses on the development of a Förster resonance energy transfer (FRET)-based "turn-on" fluorescent nanosensor for real time, sensitive detection of E.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!