In the past, ultrastructural investigations of Leishmania mexicana amastigotes revealed structures that were tentatively identified as autophagosomes. This study has now provided definitive data that autophagy occurs in the parasite during differentiation both to metacyclic promastigotes and to amastigotes, autophagosomes being particularly numerous during metacyclic to amastigote form transformation. Moreover, the results demonstrate that inhibiting two major lysosomal cysteine peptidases (CPA and CPB) or removing their genes not only interferes with the autophagy pathway but also prevents metacyclogenesis and transformation to amastigotes, thus adding support to the hypothesis that autophagy is required for cell differentiation. The study suggests that L. mexicana CPA and CPB perform similar roles to the aspartic peptidase PEP4 and the serine peptidase PRB1 in Saccharomyces cerevisiae. The results also provide an explanation for why L. mexicana CPA/CPB-deficient mutants transform to amastigotes very poorly and lack virulence in macrophages and mice.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1365-2958.2006.05274.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Correlation of local and serum CircHIPK3 expressions with the progression of liver fibrosis/cirrhosis.
Arab J Gastroenterol
January 2025
Department of Medical Imaging, Tianjin Medical University Baodi Hospital, China. Electronic address:
Background And Study Aims: This study was aimed to validate the correlation of circular RNA HIPK3 (CircHIPK3) expression in serum and tissues with the progression of liver fibrosis (LF) and liver cirrhosis (LC).
Patients And Methods: Serum CircHIPK3 expressions were detected in 120 patients with LF/LC and 120 healthy controls (HCs). CircHIPK3 expression in tissues was detected in 120 fibrotic liver tissues and compared to 57 healthy liver tissues from patients with hepatic hemangioma.
Experience With Dabigatran on Rate of Portal Vein Thrombosis Recanalization, Disease Progression and Survival.
Aliment Pharmacol Ther
January 2025
Division of Gastroenterology and Hepatology, Department of Medicine, University of Pennsylvania, Philadelphia, USA.
Background And Aims: We assessed clinical, procoagulant and genetic risk factors and clinical outcomes in dabigatran-treated patients with non-tumoural acute and acute-on-chronic portal vein thrombosis (PVT).
Methods: Patients with a new diagnosis of non-tumoural acute and acute-on-chronic PVT between January 2021 and January 2024 (aged ≥ 18 years) in those without/with cirrhosis (Child-Pugh (CP)-A/B/C ≤ 10) were started on dabigatran and followed and compared with those on vitamin K antagonist (VKA) and untreated individuals.
Results: Dabigatran was prescribed in 119 patients with PVT type 1 (61, 51.
Microbiological pathogens in fecal samples of foals during the first year of life.
Tierarztl Prax Ausg G Grosstiere Nutztiere
December 2024
Laboklin GmbH & Co. KG, Bad Kissingen, Germany.
Article Synopsis
- The study aimed to analyze how the occurrence of different infectious agents in foals with diarrhea varies by age, using fecal samples sent for testing over two years.
- Results showed that 42.9% of samples tested positive for pathogens, with Rotavirus A being the most common, particularly among foals younger than one month, where it was frequently found alongside other toxins.
- The findings affirm age dependency in pathogen occurrence but suggest that infections can happen outside the expected age range; therefore, a comprehensive diagnostic approach for diarrheic foals should be used to identify all possible pathogens and prevent further spread.
Effects of high-protein feeds on growth, free amino acid metabolism and protein metabolism-related genes in larvae and juveniles of rice flower carp (Procypris merus).
Comp Biochem Physiol Part D Genomics Proteomics
December 2024
College of Animal Science and Technology, Guangxi University, Nanning 530004, China. Electronic address:
The effect of dietary protein on fish is widely studied. However, the high-protein diet effects and mechanisms on growth and amino acid metabolism in Procypris merus remain unclear. In this study, we investigated the effect of dietary protein levels (38 %, 44 %, 50 %) on the growth performance and amino acid contents in larvae and juveniles of P.
View Article and Find Full Text PDFTherapeutic drug monitoring and safety of voriconazole in patients with liver dysfunction.
Antimicrob Agents Chemother
November 2024
Department of Pharmacy, The Affiliated Changsha Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
Article Synopsis
- This study investigates how voriconazole (VRC) levels are distributed in patients with liver dysfunction, emphasizing the need for careful monitoring of drug levels in this population.
- It analyzes medical records of 102 hospitalized patients and identifies factors like age, liver disease severity (Child-Pugh score), CRP levels, and bilirubin that affect VRC concentrations.
- The findings suggest a high rate of VRC-related side effects and propose that dosage adjustments, including therapeutic drug monitoring, are crucial for older patients and those with severe infections or low albumin levels to avoid toxicity.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!