Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
To establish a constitutive, high-efficiency expression and secretion system for Bacillus pumilus, the function of a promoter and the abilities of three signal peptides in B. pumilus DX01 were tested. F1, cloned from the rice epiphyte B. pumilus strain DX01, had strong transcription activity and was a vegetative-phase constitutive promoter. The signal sequences of Bacillus subtilis levansucrase (sacB) and subtilisin, as well as B. pumilus DX01 RNase signal sequence could drive the secretion of E. coli beta-lactamase from B. pumilus DX01 efficiently, among which the signal sequence of B. subtilis sacB was the most effective. Likewise, they could also direct the secretion of green fluorescence protein (GFP) from DX01.
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Source |
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http://dx.doi.org/10.1007/s10529-006-9030-x | DOI Listing |
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