AI Article Synopsis
- A new HPLC-UV method has been developed to measure the atypical antipsychotic quetiapine and different forms of fluvoxamine simultaneously, using a specific column and mobile phase.
- The method involves a solid-phase extraction process that requires only 300 µL of plasma and uses citalopram as an internal standard, allowing for efficient analysis.
- Validation results showed high extraction yields (over 93%), great precision (less than 4% RSD), and successful application to human plasma samples, achieving mean recovery rates exceeding 94%.
Article Abstract
An original HPLC-UV method has been developed for the simultaneous determination of the atypical antipsychotic quetiapine and the geometric isomers of the second-generation antidepressant fluvoxamine. The analytes were separated on a reversed-phase C8 column (150 mm x 4.6mm i.d., 5 microm) using a mobile phase composed of acetonitrile (30%) and a 10.5mM, pH 3.5 phosphate buffer containing 0.12% triethylamine (70%). The flow rate was 1.2 mL min(-1) and the detection wavelength was 245 nm. Sample pretreatment was carried out by an original solid-phase extraction procedure using mixed-mode cation exchange (DSC-MCAX) cartridges; only 300 microL of plasma were needed for one analysis. Citalopram was used as the internal standard. The method was validated in terms of linearity, extraction yield, precision and accuracy. Good linearity was obtained in plasma over the 5.0-160.0 ng mL(-1) concentration range for each fluvoxamine isomer and over the 2.5-400.0 ng mL(-1) concentration range for quetiapine. Extraction yield values were always higher than 93%, with precision (expressed as relative standard deviation values) better than 4.0%. The method was successfully applied to human plasma samples drawn from patients undergoing polypharmacy with the two drugs. Satisfactory accuracy values were obtained, with mean recovery higher than 94%.
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| http://dx.doi.org/10.1016/j.jchromb.2006.06.001 | DOI Listing |
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