We have prepared a rabbit antiserum specifically directed against the genome-linked protein (VPg) of cowpea mosaic virus by injecting an hydrolysate of purified virion RNA. Using this antiserum as a probe in combination with "Western" (protein) blots of subcellular fractions of cowpea mosaic virus-infected cowpea (Vigna unguiculata) cells, we have detected a bottom component RNA-encoded, 60,000-dalton polypeptide which is membrane bound and presumably represents the immediate precursor of VPg.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC256847 | PMC |
| http://dx.doi.org/10.1128/JVI.41.3.1083-1088.1982 | DOI Listing |
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