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Ferguson Plot Analysis of Chaperone ClpB from Moderate Halophile.
Protein J
January 2025
Alliance Protein Laboratories, 13380 Pantera Road, San Diego, CA, 92130, USA.
The Ferguson plot is a simple method for determining the molecular weight of native proteins and their complexes. In this study, we tested the validity of the Ferguson plot based on agarose native gel electrophoresis using multimeric chaperone protein, ClpB, derived from a moderate halophile that forms a native hexamer. The Ferguson plot showed a single band with a molecular weight of 1,500 kDa, approximately twice the size of the native hexamer.
View Article and Find Full Text PDFLeishmania mexicana N-Acetyltransferease 10 Is Important for Polysome Formation and Cell Cycle Progression.
Mol Microbiol
January 2025
Laboratório de Biologia Molecular de Patógenos (LBMP), Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo (Unifesp), São Paulo, Brazil.
Leishmania presents a complex life cycle that involves both invertebrate and vertebrate hosts. By regulating gene expression, protein synthesis, and metabolism, the parasite can adapt to various environmental conditions. This regulation occurs mainly at the post-transcriptional level and may involve epitranscriptomic modifications of RNAs.
View Article and Find Full Text PDFLeaf Dry Matter Content Is Phylogenetically Conserved and Related to Environmental Conditions, Especially Wildfire Activity.
Ecol Lett
January 2025
National Forestry and Grassland Administration Engineering Research Centre for Southwest Forest and Grassland Fire Ecological Prevention, College of Forestry, Sichuan Agricultural University, Chengdu, China.
Leaf dry matter content (LDMC) is an important determinant of plant flammability. Investigating global patterns of LDMC could provide insights into worldwide plant flammability patterns, informing wildfire management. We characterised global patterns of LDMC across 4074 species from 216 families, revealing that phylogenetic and environmental constraints influence LDMC.
View Article and Find Full Text PDFIdentification of Cannabis Product Characteristics and Pricing on Dark Web Markets.
J Psychoactive Drugs
January 2025
Global Health Policy and Data Institute, San Diego, CA, USA.
Growing cannabis use has made it the most widely cultivated and trafficked illicit drug globally according to the World Health Organization, with 147 million people consuming cannabis-derived products (CDPs) in various product forms and constituency. Despite restrictions in certain countries, unregulated access can still be found on the dark web which specializes in trafficking of illicit goods. The objective was to systematically collect data from multiple marketplaces to identify types of cannabis products offered for sale.
View Article and Find Full Text PDFCsAgBiBr and related Halide double perovskite porous single crystals.
Sci Rep
January 2025
Multifunctional Materials Laboratory, Department of Physics, Indian Institute of Technology Madras, Chennai, 600036, India.
The utilization of single crystals is exponentially growing in optoelectronic devices due to their exceptional benefits, including high phase purity and the absence of grain boundaries. However, achieving single crystals with a porous structure poses significant challenges. In this study, we present a method for fabricating porous single crystals (porous-SC) of CsAgBiBr and related halide double perovskites using an infrared-assisted spin coating technique.
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