Objectives: Malondialdehyde (MDA) as a part of thiobarbituric acid reacting substances (TBARS) is frequently used as an indicator of lipid peroxidation. Most methods for the measurement of TBARS require long derivatization time and addition of antioxidants in the samples. Furthermore, comparison of these methods with commercially available HPLC kits is lacking.
Design And Methods: We investigated column performance of five different columns, tested eight different acids for the hydrolysis of the samples, and estimated stability of derivatized plasma samples with different anticoagulants. The samples were derivatized with TBA. The peak for the TBA(2)-MDA adduct was separated and detected by HPLC.
Results: Performance of the Phenomenex Gemini column was best. PCA at the concentration of 0.1125 N was used in this method. Coefficient of variation (CV %) within the run and between the run was 4.1% and 6.7%, and analytical recovery was 90-94%. The retention time of the TBA(2)-MDA peak was 1.8 min. Reference intervals for TBARS in serum from 250 individuals were 0.53 and 2.1 micromol/l using our HPLC method and 0.07 and 0.24 micromol/l using the Chromsystems assay. Linear regression with log converted values revealed weak relationship between the two methods (r(2) = 0.064).
Conclusions: Our HPLC method for the analysis of TBARS in serum and plasma is fast and accurate and therefore can be used in clinical studies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.clinbiochem.2006.03.012 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Synthesis of a fluorophilic magnetic microporous organic network for selective enrichment of fipronil and ethiprole in milk and egg samples.
J Chromatogr A
January 2025
State Key Laboratory of Advanced Drug Delivery and Release Systems, and Medical Science and Technology Innovation Center, School of Pharmaceutical Sciences & Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China. Electronic address:
Considering the widespreadly use, large consumption, and serious environmental and health threats of phenylpyrazole insecticides (PPIs), development of a selective and sensitive method for accurate detection of their residuals in food samples is of great significance and challenging. Herein, depending on the hydrophobic and F-containing characteristics of PPIs, a novel fluorinated magnetic microporous organic network (FMMON) was designed and prepared for efficient and selective magnetic solid-phase extraction (MSPE) of two typical PPIs (fipronil and ethiprole) from milk and egg samples before the HPLC-UV determination. FMMON owned large specific surface area, multiple interaction sites, excellent magnetic separation performance and stability and exhibited good extraction and selectivity for fipronil and ethiprole through the specific F-F, hydrogen bonding, hydrophobic, and π-π interactions.
View Article and Find Full Text PDFDevelopment of a novel HPLC-HRMS method for quantitative analysis of resorcinol in urine: Application to hairdressers' occupational exposure.
J Chromatogr B Analyt Technol Biomed Life Sci
January 2025
Resorcinol is a widespread substance used in a large variety of manufacturing industries, including cosmetics, with endocrine-disrupting activity on the thyroid function. The aim of the present study was to develop and validate a sensitive, selective and robust method to quantify resorcinol in urine and thereby assess hairdressers' occupational exposure. As resorcinol is mainly excreted in urine as glucuronide or sulfate forms, the first step consisted in hydrolyzing urine samples with a β-glucuronidase-arylsulfatase enzyme for 16 h.
View Article and Find Full Text PDFDevelopment and validation of a high-performance liquid chromatography method with fluorescence detection for the quantification of the resistance protein P-gp in cancer cells.
J Chromatogr B Analyt Technol Biomed Life Sci
January 2025
Université Clermont Auvergne, Institut Universitaire de Technologie, UMR INSERM-UCA, U1240, Imagerie Moléculaire et Stratégies Théranostiques (IMoST), 5 Avenue Blaise Pascal, 63000 Clermont-Ferrand, France.
A method using high-performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) was developed and validated to quantify the innovative tool LightSpot®-FL-1, a selective permeability-glycoprotein (P-gp)-targeted fluorescent conjugate used to measure P-gp expression in cell samples. Quantifying P-gp is a major challenge in oncology as its overexpression in many cancer cells results in Multidrug Resistance (MDR) associated with chemotherapy failure. To develop the method reported herein, both sample preparation and analysis parameters were investigated.
View Article and Find Full Text PDFPhytochemical composition, antioxidant and antimicrobial activities of Delile ex Godr flowers extracts.
Nat Prod Res
January 2025
Laboratory of Organic Chemistry LR17-ES08 (Natural Substances Team), Faculty of Sciences of Sfax, University of Sfax, Sfax, Tunisia.
The phytochemical profile of various plant species reveals that some compounds possess notable antioxidant and antimicrobial properties. In this study we investigated for the first time, the antioxidant activity (FRAP, DPPH and TAC), total phenolic contents and total flavonoid contents of Delile ex Godr flowers extracts (-hexane, ethyl acetate and methanol) as well as their antimicrobial activity. The results obtained showed that the methanol extract contained the highest content of total phenolics (346.
View Article and Find Full Text PDFPrilling as an Effective Tool for Manufacturing Submicrometric and Nanometric PLGA Particles for Controlled Drug Delivery to Wounds: Stability and Curcumin Release.
Pharmaceutics
January 2025
Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, Italy.
Background/objectives: This study investigates for the first time the use of the prilling technique in combination with solvent evaporation to produce nano- and submicrometric PLGA particles to deliver properly an active pharmaceutical ingredient. Curcumin (CCM), a hydrophobic compound classified under BCS (Biopharmaceutics Classification System) class IV, was selected as the model drug.
Methods: Key process parameters, including polymer concentration, solvent type, nozzle size, and surfactant levels, were optimized to obtain stable particles with a narrow size distribution determined by DLS analysis.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!