Objective: To study the chemical constituents of Mentha spicata.
Method: The chemical constituents were isolated by silica gel column chromatography, and identified by physical and chemical characters and spectroscopic analysis.
Result: Compounds I - V were obtained and their structures were elucidated as protocatechuic aldehyde (I), protocatechuic acid (II), chrysoeriol (III), 5, 6-dihydroxy-7, 8, 3', 4'-tetramethoxyflavone (IV), nodifloretin (V).
Conclusion: Compound I and II were first isolated from the genus Mentha. Compound Ill, IV and V were isolated from M. spicata for the first time.
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