[Expression of HIC-5/ARA55 in colonrectal cancer and its mechanisms of action].

Objective: To investigate the relationship between HIC-5 and colonrectal cancer.

Methods: Samples were obtained from 42 patients with colonrectal cancer, and the mRNA and protein expressions of HIC-5/ARA55 were determined by RT-PCR and Western-blot respectively. FCM was used to investigate the apoptosis of Lovo in the HIC-5/ARA55 transfection cells. The expression of apoptosis related proteins Bax and Bcl-2 was determined by Western-blot, and the MTT assay was used to test cell growth speed affected by HIC-5/ARA55.

Results: The expression of HIC-5 significantly decreased on colonrectal cancer tissues as compared with adjacent normal tissues on mRNA level and protein level(P<0.05). The apoptosis rate increase significantly in the HIC-5/ARA55 transfected cells. HIC-5/ARA55 could restrain tumor cell grow speed.

Conclusion: HIC-5/ARA55 is the protein that can reduce tumor cell growth rate, and induce apoptosis in colonrectal cancer cells.