Reversed-phase high-performance liquid chromatography (RP-HPLC) with UV detection at 210 nm was used to monitor the formation of the major compounds during the lipase-catalyzed transesterification reaction of sunflower oil with methanol. Individual triacylglycerols, diacylglycerols, monoacylglycerols as well as fatty acids and their corresponding methyl esters were separated using acetonitrile/acetone as a mobile phase and a combined linear gradient-isocratic-step gradient-isocratic elution procedure. Another relatively short method consisting of a linear gradient elution followed by an isocratic elution gave similar results, yet with lower resolution. HPLC/mass spectrometry with an ion trap analyzer and atmospheric pressure chemical ionization source was used for the identification of the individual compounds. Individual calibration curves obtained with UV detection at 210 nm were found to be of use for quantitative analyses of double-bond containing methyl esters and acylglycerols. The use of the RP-HPLC methods in the elucidation of the mechanisms of three immobilized lipases, namely Lipozyme TL IM, Lipozyme RM IM and Novozym 435, in biodiesel production was described.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.chroma.2006.05.065 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fabrication and characterization of non-diary whipped creams: Influence of oleogel.
Food Chem
January 2025
College of Food Science, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China. Electronic address:
Non-dairy whipped creams (NDWC) are a typical food emulsion system and are gaining popularity among consumers. Oleogels as reasonable alternatives to trans and saturated fats in foods show great potential application in NDWC. Effects of different proportions of oleogel (30 %-70 %) as base oil on the crystallization behavior, appearance, interface and rheological properties of NDWC were evaluated.
View Article and Find Full Text PDFRole of Seed Therapy on Estrous and Non-Estrous Cycle in Healthy Female Rats.
Food Sci Nutr
January 2025
Seed cycling therapy (SCT) involves the consumption of specific seeds during the follicular and luteal phases of the menstrual cycle to help balance reproductive hormones. This study aimed to investigate the effects of SCT on healthy female Wistar albino rats to prevent hormonal imbalances. For SCT, a seed mixture (SM1) consisting of flax, pumpkin, and soybeans (estrogenic seeds) was administered at doses of 5.
View Article and Find Full Text PDFCrystallized Pickering Emulsions from Plant Oil as a Local Alternative to Palm Oil.
Foods
January 2025
Institute of Food and Beverage Innovation, Zurich University of Applied Sciences, Einsiedlerstrasse 35, 8820 Wädenswil, Switzerland.
Palm and palm kernel oils are preferred ingredients in industrial food processing for baked goods and chocolate-based desserts due to their unique properties, such as their distinctive melting behaviors. However, ongoing concerns about the social and environmental sustainability of palm oil production, coupled with consumer demands for palm oil-free products, have prompted the industry to seek alternatives which avoid the use of other tropical or hydrogenated fats. This project investigated replacing palm oils with chemically unhardened Swiss sunflower or rapeseed oils.
View Article and Find Full Text PDFThe Effect of the Addition of Silicon Dioxide Particles on the Tribological Performance of Vegetable Oils in HCT600X+Z/145Cr46 Steel Contacts in the Deep-Drawing Process.
Materials (Basel)
December 2024
Institute of Technology and Materials Engineering, Technical University of Košice, Mäsiarska 74, 040 01 Košice, Slovakia.
Friction is an unfavourable phenomenon in deep-drawing forming processes because it hinders the deformation processes and causes deterioration of the surface quality of drawpieces. One way to reduce the unfavourable effect of friction in deep-drawing processes is to use lubricants with the addition of hard particles. For this reason, this article presents the results of friction tests of dual-phase HCT600X+Z steel sheets using the flat die strip drawing test.
View Article and Find Full Text PDFComparison of Coconut and Sunflower Seed Oils in Improving the Skin Integrity and Weight Gain of Infants in the Neonatal ICU.
Adv Skin Wound Care
January 2025
Öznur Tiryaki, PhD, RN, is Associate Professor, Faculty of Health Sciences, Department of Midwifery, Sakarya University, Sakarya, Turkey. Hamide Zengin, PhD, RN, is Associate Professor, Faculty of Health Science, Department of Pediatric Nursing, Eskişehir Osmangazi University, Eskişehir, Turkey. Also at Sakarya University, Nursan Çınar, PhD, RN, is Professor, Faculty of Health Sciences, Department of Pediatric Nursing; Meltem Karabay, MD, is Associate Professor, Faculty of Medicine, Research and Training Hospital of Sakarya, Division of Neonatology, Department of Pediatrics; İbrahim Caner, MD, is Professor, Faculty of Medicine, Research and Training Hospital of Sakarya, Division of Neonatology, Department of Pediatrics; and Ertuğrul Güçlü, MD, is Professor, Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology.
Objective: To determine the effects of sunflower seed oil and coconut oil on the skin integrity and weight gain of preterm infants in the neonatal ICU.
Methods: In this randomized controlled trial, 66 preterm neonates (34-37 weeks' gestation) in the neonatal ICU of a training and research hospital were equally divided into three groups: sunflower seed oil, coconut oil, and control. The weights of neonates in all three groups were measured at admission to the neonatal ICU, at discharge, and at 1 month postdischarge.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!