Visualization of human sperm chromosomes by using mouse oocytes cryothawed after enucleation.

Objective: To evaluate whether previously enucleated mouse oocytes that are vitrified are usable for the analysis of human sperm chromosomes.

Design: Prospective, comparative laboratory study.

Setting: Animal breeding and reproduction department in a rural Japan university.

Patient(s): Sperm samples were obtained from a fertile donor.

Intervention(s): Human sperm were injected into previously enucleated-vitrified mouse oocytes.

Main Outcome Measure(s): The rates of appearance of the human sperm chromosomes that were injected into previously enucleated-vitrified mouse oocytes.

Result(s): The rates of appearance of human sperm chromosomes were 71%-72% in enucleated-vitrified mouse oocytes.

Conclusion(s): The use of enucleated-vitrified mouse oocytes allowed visualization of human sperm chromosomes.