Background: The usual criteria for analysis of hepatitis B surface antigen (HBsAg) are detection of HBsAg and result confirmation by antibody neutralization. We observed that with the Immulite 2000 HBsAg assay [Diagnostics Product Corporation (DPC)] a relatively high percentage of weakly reactive (WR) samples did not pass the neutralization step.
Methods: For each of 3 lots of Immulite 2000 HBsAg reagent (DPC), we collected and analyzed HBsAg data from approximately 3000 to 4000 patient blood samples and compared these data with HBsAg data from 3393 samples tested with the Abbott Auszyme assay. For 127 samples with initially WR detection signals (relative signal/cutoff index of 1.00-2.5) on the Immulite 2000 HBsAg assay, we then measured hepatitis B (HB) viral load and/or other HB serologic markers.
Results: The Immulite 2000 HBsAg assay produced more initially reactive results than the Abbott Auszyme method. Many of these reactive samples, however, were WR and did not meet the confirmation criteria in the neutralization test. Moreover, DNA PCR testing indicated that 22 of the 38 WR samples (58%) that did meet the confirmation criteria had no detectable HB viral DNA.
Conclusions: Immulite 2000 HBsAg assay results include a unique group of WR samples that are associated with both false-positive and false-negative results, regardless of neutralization status, and require careful interpretation. WR HBsAg samples should be reported as confirmed HBsAg reactive only if the samples not only meet the neutralization criteria but also are positive for other HB serologic markers such as anti-HB core total and anti-HB core IgM.
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http://dx.doi.org/10.1373/clinchem.2005.064063 | DOI Listing |
Transpl Immunol
December 2024
Department of Medical Immunology, Beni-Messous Teaching Hospital, Algiers, Algeria; Faculty of Pharmacy, University of Algiers, Algiers, Algeria.
Introduction: Cytomegalovirus (CMV) is a virus of the herpesviridae family. CMV infection is associated with increased morbidity and mortality in immunocompromised subjects such as hemodialysis patients and transplant recipients. The aim of our study was to determine the serological status of potential kidney recipients and donors in order to assess the risk of post-transplant CMV infection and disease.
View Article and Find Full Text PDFClin Nutr
January 2025
Department of Nutritional Sciences, University of Toronto, Toronto ON, M5S 1A8, Canada; Translational Medicine, The Hospital for Sick Children, Toronto ON, M5G 0A4, Canada; Rogers Hixon Ontario Human Milk Bank, Sinai Health, Toronto ON, M5G 1X5, Canada; Department of Pediatrics, Sinai Health, Toronto ON, M5G 1X5, Canada. Electronic address:
Background & Aims: Feeding parent's milk with supplemental donor milk (DM) is the optimal way to feed very low birth weight (VLBW) infants instead of formula; however, suboptimal neurodevelopment persists. This is believed due, in part, to suboptimal nutrition. Given vitamin B12's role in neurodevelopment and increased adoption of plant-based diets among females of child-bearing age, we aimed to determine the adequacy of vitamin B12 in DM (n = 380 donors) and associated donor characteristics.
View Article and Find Full Text PDFHeliyon
October 2024
Department of Laboratory Medicine, Yongin Severance Hospital, Yonsei University College of Medicine, Yongin, South Korea.
J Vet Cardiol
December 2024
Lamb Statistical Consulting LLC, West Saint Paul, MN, USA.
J Clin Lab Anal
September 2024
Pediatric Research Center, New Children's Hospital, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Background: In our recent publications, we reported the identification of three different molecular forms of total luteinizing hormone (LH) in urine, the intact LH, the free beta-subunit (LHβ), and its core fragment of LHβ (LHβcf), the latter two establishing the nonintact portion of LH. Following the discontinuation of the Delfia immunofluorometric assay (IFMA) (Wallac, PerkinElmer Finland, Finland), a leading method for detecting urinary LH for 30 years, this study seeks to assess the efficacy of three alternative commercial immunoassays in identifying various forms of U-LH.
Methods: Diluted urine samples underwent gel filtration to separate them into fractions, each containing different forms of LH.
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